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Engineering of Self-assembly Polymers Encapsulated with Dual Anticancer Drugs for the Treatment of Endometrial Cancer

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Abstract

Chemotherapy side effects are minimized, and therapeutic results are enhanced by combination therapy, which is extensively utilized in cancer therapy. MTT experiment showed that compared to the free drugs calreticulin (CRT) and gemcitabine (GEM), GEM-CRT@NPs significantly increased the cytotoxicity of endometrial cancer (Ishikawa cells and KLE cells). These drugs were conjugated to a tri-block copolymeric framework. GEM-CRT@NPs were studied using HR-TEM and DLS analysis to determine particle size and zeta potential. Biochemical staining analyses were also used to investigate the morphological characteristics of endometrial cancer cell lines. After KLE and Ishikawa cancer cells were treated with GEM-CRT@NPs, endometrial cell shape was altered, as evidenced by nuclear fragmentation and nuclear condensation (apoptosis hallmarks). Additionally, the most significant apoptosis ratio to mitochondrial membrane potential indicates that the mitochondrial membrane potential is responsible for the cell's death. Upon treatment with GEM-CRT@NPs, systemic toxicity analysis revealed minimal histological abnormalities and significant blood chemistry alterations, and a near-normal look of the organs. Endometrial cancer treatment may be enhanced with the use of the targeted combination therapeutic system.

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  1. Department of Pathology, Nantong Maternal and Child Health Hospital, No. 399, Century Avenue, Chongchuan District, Nantong, 226006, Jiangsu Province, China

    Ting-ting Chen, Ming-ming Yuan, Yu-mei Tao & Xiao-yan Ren

  2. Department of Gynecology, the First Hospital of Shanxi Medical University, No.85, Jiefang South Road, Taiyuan, 030000, Shanxi Province, China

    Sufen Li

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Chen, Tt., Yuan, Mm., Tao, Ym. et al. Engineering of Self-assembly Polymers Encapsulated with Dual Anticancer Drugs for the Treatment of Endometrial Cancer. J Clust Sci 33, 2661–2671 (2022). https://doi.org/10.1007/s10876-021-02175-5

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