Abstract
Due to their excellent mechanical strength and biocompatibility, silk fibroin(SF) hydrogels can serve as ideal scaffolds. However, their slow rate of natural degradation limits the space available for cell proliferation, which hinders their application. In this study, litchi-like calcium carbonate@hydroxyapatite (CaCO3@HA) porous microspheres loaded with proteases from Streptomyces griseus (XIV) were used as drug carriers to regulate the biodegradation rate of SF hydrogels. The results showed that litchi-like CaCO3@HA microspheres with different phase compositions could be prepared by changing the hydrothermal reaction time. The CaCO3@HA microspheres controlled the release of Ca ions, which was beneficial for the osteogenic differentiation of mesenchymal stem cells (MSCs). The adsorption and release of protease XIV from the CaCO3@HA microcarriers indicated that the loading and release amount can be controlled with the initial drug concentration. The weight loss test and SEM observation showed that the degradation of the fibroin hydrogel could be controlled by altering the amount of protease XIV-loaded CaCO3@HA microspheres. A three-dimensional (3D) cell encapsulation experiment proved that incorporation of the SF hydrogel with protease XIV-loaded microspheres promoted cell dispersal and spreading, suggesting that the controlled release of protease XIV can regulate hydrogel degradation. SF hydrogels incorporated with protease XIV-loaded microspheres are suitable for cell growth and proliferation and are expected to serve as excellent bone tissue engineering scaffolds.
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Acknowledgements
The National Natural Science Foundation of China (11532004, 51603026); the Natural Science Foundation Project of CQ (cstc2018jcyjAX0711, cstc2018jcyjAX0286). Chongqing Technology Innovation and Application Development Project (cstc2019jscx-msxmX0231). Chongqing University of Science and Technology Graduate Science and Technology Innovation Project (YKJCX1920213).
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10856_2020_6466_MOESM1_ESM.tif
Figure S1 Digital images of SF hydrogel incorporated with different amounts of protease XIV-loaded litchi-like CaCO3@HA microspheres at different coculture time. (1, 2, 3 represent S0, S5, S10, respectively; a, b, c, d represent 0d, 1d, 3d, 5d, respectively)
10856_2020_6466_MOESM2_ESM.tif
Figure S2 Digital images of SF hydrogel incorporated with different amounts of protease XIV-loaded litchi-like CaCO3@HA microspheres before and after compressive strength test. (a, b, c represent S0, S5, S10, respectively)
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Xiao, W., Zhang, J., Qu, X. et al. Fabrication of protease XIV-loaded microspheres for cell spreading in silk fibroin hydrogels. J Mater Sci: Mater Med 31, 128 (2020). https://doi.org/10.1007/s10856-020-06466-7
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DOI: https://doi.org/10.1007/s10856-020-06466-7