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Development and preparation of a low-immunogenicity porcine dermal scaffold and its biocompatibility assessment

  • Biocompatibility Studies
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Abstract

Acellular dermal matrix (ADM) has been widely used in repair and reconstruction of tissue defect. Therapeutic effect of porcine ADM (PADM) is inferior to that of human ADM (HADM). Relatively high immunogenicity and the resulting strong inflammatory response are major issue in application of PADM. We therefore treated reticular layer PADM (Rl-PADM) with matrix metalloproteinase-7 (MMP-7) and obtained a low-immunogenicity porcine dermal scaffold (LIPDS). Highly immunogenic components, tissue structure, cytocompatibility, and postgrafting histological changes of LIPDS were further investigated. Compared with Rl-PADM, LIPDS showed that the epithelial root sheath, cell debris, laminin, and type IV collagen were almost entirely removed, the structure remained normal, and the interfibrous space was relatively enlarged. Cytocompatibility of LIPDS was similar to that of HADM but superior to Rl-PADM. With regard to the extent of tissue ingrowth in terms of host fibroblasts infiltration and vascularization, LIPDS exhibited clear advantages over Rl-PADM after they had been subcutaneously transplanted in a rat model. In addition, no excessive inflammatory response was observed in LIPDS group up to 28 days postgraft, and the morphosis of collagenous fibers kept essentially normal. However, there were stronger inflammatory response and obvious collagen spallation in Rl-PADM group. The processes of integration and remodeling after the LIPDS grafting were similar to those of a normal wound healing response. The LIPDS graft was vascularized at a relatively high speed. Thus, as an implantable scaffold material, LIPDS is a superior template for guiding tissue regeneration and remodeling.

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Acknowledgments

This study was supported in part by grants from Building Project on National Clinical Key Specialty of China ([2012]649), Science and Technology Development Project of Shangdong Province (2013GSF11870), Science and Technology Development Project of Shangdong Province Health and Medicine (2011HZ008), and Business Project of Study Abroad Returnees in Jinan (20080405).

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Authors and Affiliations

  1. Department of Burns, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan, 250013, Shandong, People’s Republic of China

    Guodong Song, Fang Wang, Yang Shao, Chunjie Fan, Peilong Li, Yonghu Zhang & Haibin Zuo

  2. Medical Image Center, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan, 250013, Shandong, People’s Republic of China

    Yan Wu

  3. Department of Burns and Plastic Surgery, Hefei First People’s Hospital, The Third Affiliated Hospital of Anhui Medical University, 390 Huaihe Road, Hefei, 230000, Anhui, People’s Republic of China

    Jinzhu Jiang

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  1. Guodong Song
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  2. Yan Wu
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  8. Yonghu Zhang
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  9. Haibin Zuo
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Correspondence to Guodong Song.

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Song, G., Wu, Y., Wang, F. et al. Development and preparation of a low-immunogenicity porcine dermal scaffold and its biocompatibility assessment. J Mater Sci: Mater Med 26, 170 (2015). https://doi.org/10.1007/s10856-015-5503-6

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  • DOI: https://doi.org/10.1007/s10856-015-5503-6

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