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Development of amphotericin B-loaded fibroin nanoparticles: a novel approach for topical ocular application

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Abstract

Fungal keratitis (FK), the major cause of ocular morbidity worldwide, is commonly treated with the amphotericin B (AmB) eye drops extemporaneously prepared from marketed parenteral formulations. However, these in-house prepared AmB eye drops have the drawbacks of poor ocular bioavailability and eye irritation. The aim of this study was to develop AmB-loaded fibroin nanoparticles (AmB-FNPs), in combination with the polymer PEG 400, as ready-to-use eye drops for FK. The AmB-FNPs were prepared by desolvation method. All AmB-FNPs exhibited homogeneous spherical particles with a mean size of ~ 270 nm, the zeta potential of ~ − 17 mV, and an entrapment efficiency of ~ 65%. Using X-ray diffraction and UV analysis, AmB demonstrated amorphous molecular dispersion and monomeric form when entrapped in the FNPs. Interestingly, in dissolution studies, although AmB-FNPs showed no detectable drug release in sink condition, they still possessed good antifungal activity against Candida albicans. Potentially, AmB-FNPs showed less cytotoxicity in human corneal epithelial cell line compared to the marketed AmB deoxycholate.

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Acknowledgements

The author would like to thank Miss Manisha Bangar of UCL School of Pharmacy, University College London for her assistance in conducting the experiments.

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Authors and Affiliations

  1. College of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand

    Pratthana Chomchalao

  2. Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, 65000, Thailand

    Pratthana Chomchalao, Pataranapa Nimtrakul, Duy Toan Pham & Waree Tiyaboonchai

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  1. Pratthana Chomchalao
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  2. Pataranapa Nimtrakul
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Correspondence to Waree Tiyaboonchai.

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Chomchalao, P., Nimtrakul, P., Pham, D.T. et al. Development of amphotericin B-loaded fibroin nanoparticles: a novel approach for topical ocular application. J Mater Sci 55, 5268–5279 (2020). https://doi.org/10.1007/s10853-020-04350-x

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