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High sensitivity detection of human serum albumin using a novel magnetoelastic immunosensor

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Abstract

In this paper, a novel and wireless magnetoelastic (ME) immunosensor for human serum albumin (HSA) detection is presented. Gold nanoparticles layer on the sensor surface can enhance its biocompatibility, stability and sensitivity. The anti-HSA IgG, as a capture probe, was immobilized on the gold-plated ME sensor surface to specifically recognize the target HSA. The binding complex accumulated on the immunosensor surface leads to a decrease in the immunosensor’s resonance frequency, corresponding to the HSA concentrations. The atomic force microscopy, the Raman spectrum and the X-ray electron spectroscopy were used to confirm that the antibody immobilization was successful. Furthermore, to enhance its sensitivity, the working concentration of the antibody was optimized to be 25 µg ml−1. The experimental results demonstrated that the immunosensor exhibited a linear response to the logarithm of HSA concentrations ranging from 0.01 to 100 µg ml−1, with the sensitivity of 9.3 Hz/μg ml−1 and the detection limit of 0.01 µg ml−1, which is significantly lower than the minimum diagnosis limit of urine microalbumin.

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Acknowledgements

The authors are grateful for the financial support from the National Natural Science Foundation of China (Nos. 51622507, 61471255, 61474079, 61501316, 51505324).

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Author notes

  1. Rong Liu and Xing Guo have contributed equally to this work.

Authors and Affiliations

  1. MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education and College of Information and Computer, Taiyuan University of Technology, Jinzhong, 030600, China

    Rong Liu, Xing Guo, Jingzhe Wang, Jinyu Guo, Yixia Zhang, Wendong Zhang & Shengbo Sang

  2. Institute of Applied Mechanics and National Demonstration Center for Experimental Mechanics Education, College of Mechanical and Vehicle Engineering of Taiyuan University of Technology, Jinzhong, 030600, China

    Yixia Zhang

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  1. Rong Liu
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  2. Xing Guo
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Correspondence to Shengbo Sang.

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Liu, R., Guo, X., Wang, J. et al. High sensitivity detection of human serum albumin using a novel magnetoelastic immunosensor. J Mater Sci 54, 9679–9688 (2019). https://doi.org/10.1007/s10853-019-03554-0

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  • DOI: https://doi.org/10.1007/s10853-019-03554-0

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