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Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization

  • Biomaterials
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Abstract

Micron-sized flower-like porous Fe3O4 microparticles were fabricated by improved solvothermal method. Then, flexible dendrimer-grafted Fe3O4@SiO2–NH2/PAMAM microcarriers were obtained by functionalized modification of Michael addition and amidation process. The immobilization of penicillin G acylase on the carrier was realized by cross-linking of glutaraldehyde, and we investigated the optimum immobilization conditions. Under the optimal immobilized conditions, the reaction activity could reach 3098.96 U/g. When the reaction temperature was 47 °C, the immobilized PGA had the highest activity of 394.74 U/g, which indicated that the immobilized PGA had better thermal stability than free PGA. The activity of immobilized enzyme maintained 82.14% after reused for nine times, which indicated that the immobilized enzyme had good stability and good industrial application potential.

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Acknowledgement

The authors are grateful for the support from the Key Program of the National Natural Science Foundation of China (No. 51433008), the Seed Foundation of Innovation and Creation for Graduate Students in Northwestern Polytechnical University (Z2017211), the Excellent Doctorate Foundation of Northwestern Polytechnical University, and the Innovation Foundation for Doctorate Dissertation of Northwestern Polytechnical University (CX201623).

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Authors and Affiliations

  1. Department of Applied Chemistry, School of Natural and Applied Sciences, Northwestern Polytechnical University, Xi’an, 710072, China

    Xue Li, Lei Tian, Zafar Ali, Wenyan Wang & Qiuyu Zhang

  2. The Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Northwestern Polytechnical University, Youyi Road 127#, Xi’an, 710072, China

    Xue Li, Lei Tian, Zafar Ali, Wenyan Wang & Qiuyu Zhang

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  1. Xue Li
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  2. Lei Tian
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  3. Zafar Ali
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  4. Wenyan Wang
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Correspondence to Qiuyu Zhang.

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Li, X., Tian, L., Ali, Z. et al. Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization. J Mater Sci 53, 937–947 (2018). https://doi.org/10.1007/s10853-017-1581-9

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  • DOI: https://doi.org/10.1007/s10853-017-1581-9

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