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Dense diamond nanoneedle arrays for enhanced intracellular delivery of drug molecules to cell lines

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Abstract

Nanotechnologies for intracellular delivery are of great value in clinical and biological research. Diamond nanoneedle arrays are a novel and attractive platform to facilitate drug delivery with minimal cytotoxicity. Using our technique, the cellular membranes can be temporarily disrupted for enhanced diffusion of drug molecules to cytoplasm. Herein we show that this technique is applicable to deliver different types of anticancer drugs into a variety of cell lines, although the membrane of each cell line possesses varied rigidity and hardness and each drug has its own unique properties and targets. When anticancer drugs and nanoneedle arrays are collaboratively used to treat cancer cells, the cell viability dramatically decreases by up to 40 % in comparison with the cells treated with drugs only. Attractively, therapeutic molecules can be efficiently delivered to drug-resistant cells with the aid of nanoneedle arrays. The combination of diamond nanoneedle arrays and anticancer drug cisplatin can decrease the viability of A549 cisplatin-resistant cells to about 60 %, while the cells only treated with the same concentration of drug are essentially not affected due to their drug resistance. These results indicate that dense nanoneedle arrays represent an effective approach to enhance the delivery of biological molecules to different types of cells. Such approach will certainly be beneficial to microbiological research and clinical applications in the future.

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    Authors and Affiliations

    1. School of Chemistry and Forensic Sciences, Faculty of Life Sciences, University of Bradford, Bradford, UK

      Xianfeng Chen

    2. Department of Physics and Materials Science, City University of Hong Kong, Kowloon, Hong Kong, SAR

      Xiaoyue Zhu, So Ying Kwok, Muk Fung Yuen, Li Yan, Wei Chen, Yang Yang, K. N. Yu & Wenjun Zhang

    3. Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, Kowloon, Hong Kong, SAR

      Xiaoyue Zhu, So Ying Kwok, Muk Fung Yuen, Li Yan, Wei Chen, Yang Yang & Wenjun Zhang

    4. Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong, SAR

      Zhigang Wang & Guangyu Zhu

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    1. Xiaoyue Zhu
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    2. So Ying Kwok
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    3. Muk Fung Yuen
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    4. Li Yan
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    5. Wei Chen
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    Corresponding authors

    Correspondence to Guangyu Zhu, Wenjun Zhang or Xianfeng Chen.

    Additional information

    Xiaoyue Zhu and So Ying Kwok have contributed equally to this work.

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    Zhu, X., Kwok, S.Y., Yuen, M.F. et al. Dense diamond nanoneedle arrays for enhanced intracellular delivery of drug molecules to cell lines. J Mater Sci 50, 7800–7807 (2015). https://doi.org/10.1007/s10853-015-9351-z

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    • DOI: https://doi.org/10.1007/s10853-015-9351-z

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