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Calix protected gold nanobeacon as turn-off fluorescent sensor for phenylalanine

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Abstract

A new calix platform i.e. resorcinarene tetra hydrazide (RTH) has been synthesized and duly characterized by IR, NMR and ESI–MS. Only one agent i.e. RTH has been used as reducing as well as stabilizing agent for the synthesis of gold nanoparticles (AuNps). The synthesised RTH-AuNPs were characterized and analyzed by UV/Vis-spectroscopy, transmission electron microscopy and energy dispersive X-ray analysis. RTH-AuNps were checked for their stability at different pH and temperature. RTH-AuNps having characteristic surface plasmon resonance and being fluorescent in nature were explored for their interaction behavior with different amino acids (AA) by UV–Visible and fluorescence spectroscopy. Among various AA RTH-AuNps were found to be meticulously selective and sensitive for phenylalanine (PHE) by means of fluorescence quenching. This assay allowed rapid and accurate determination of PHE in aqueous medium at room temperature with a linear range of detection from 100 to 820 nM. Furthermore, the RTH-AuNps were also used for successful determination of PHE in human serum providing a scope of detection and determination of PHE in biological samples.

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Abbreviations

AuNps:

Gold nanoparticles

RTH:

Resorcinarene tetrahydrazide

RTH-AuNps:

Resorcinarene tetrahydrazide stabilized gold nanoparticles

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Acknowledgments

The authors gratefully acknowledge the financial assistance provided by University Grant Commission (New Delhi). The authors also acknowledge Sophisticated Analytical Instrument Facility (Panjab University), Central Salt & Marine Chemicals Research Institute (Bhavanagar) and Gujarat Forensic Sciences University (Gandhinagar) for providing instrumental facilities and Information and Library Network (Ahmedabad) for e-journals.

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Authors and Affiliations

  1. Department of Chemistry, School of Sciences, Gujarat University, Ahmedabad, 380009, India

    Divya R. Mishra, Savan M. Darjee, Keyur D. Bhatt, Krunal M. Modi & Vinod K. Jain

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  1. Divya R. Mishra
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  2. Savan M. Darjee
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  3. Keyur D. Bhatt
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Correspondence to Vinod K. Jain.

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Mishra, D.R., Darjee, S.M., Bhatt, K.D. et al. Calix protected gold nanobeacon as turn-off fluorescent sensor for phenylalanine. J Incl Phenom Macrocycl Chem 82, 425–436 (2015). https://doi.org/10.1007/s10847-015-0509-8

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  • DOI: https://doi.org/10.1007/s10847-015-0509-8

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