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Differential sperm proteomic profiles according to pregnancy achievement in intracytoplasmic sperm injection cycles: a pilot study

  • Gamete Biology
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Abstract

Purpose

To describe the proteomic profiles in semen samples and define the differences in sperm proteomic profiles among samples that ultimately achieved pregnancy (P) via intracytoplasmic sperm injection (ICSI) in an oocyte donation program and those that were unsuccessful (NP).

Methods

Prospective, analytical, observational nested case and control study evaluating the proteomic profile of spermatozoa from patients’ ejaculates where pregnancies were (group pregnant (P), n= 4) or were not (group non-pregnant (NP), n=4) achieved after ICSI in an oocyte donation program aiming to standardize female factor. Proteins were separated and analyzed by means of SWATH-MS) and compared between P/NP groups to identify sperm biomarkers of fertility/infertility. Proteins are available via ProteomeXchange.

Results

We identified and quantified 2228 proteins, with 37 significantly higher in the P group and 16 higher in NP. Enrichment analysis revealed that the increased proteins in P group sperm were related to motility, anaerobic metabolism, and protein biosynthesis functions, while the increased proteins in the NP group were involved in protein biosynthesis, protein folding, aerobic metabolism, and signal transduction, all of which are functions not previously described as influencing sperm success. Some proteins identified (e.g., SLC2A3, or CD81) are located in the cell membrane and thus may be employed to select spermatozoa by magnetic-activated cell sorting (MACS).

Conclusion(s)

This work revealed differences in the proteomic profiles of sperm samples successful in achieving pregnancy and those that were not, expanding our understanding of sperm function and infertility-related molecular markers, and enabling the future development of male fertility diagnostic tools and therapies.

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Acknowledgements

We would like to thank the proteomics facility of SCSIE University of Valencia for the LC-MS/MS and SWATH-MS proteomic analysis. This proteomics laboratory is a member of Proteored, PRB3, and is supported by grant PT17/0019, of the PE I+D+i 2013–2016, funded by ISCIII and ERDF. We also would like to thank the statistics section of the SCSIE University of Valencia for the statistical analysis.

Funding

This study is supported by CDTI and Fondo Tecnológico. F.D participation was supported by the Spanish Ministry of Economy and Competitiveness through the Miguel Servet programme (CP013/0450) co-founded by FEDER.

Author information

Author notes

  1. Nicolas Garrido and Francisco Dominguez contributed equally to this work.

Authors and Affiliations

  1. Andrology Laboratory and Sperm Bank, IVIRMA Valencia, Plaza de la Policía Local, 3, 46015, Valencia, Spain

    Rocio Rivera-Egea & Nerea Sota

  2. IVI Foundation, Health Research Institute La Fe, Edificion Biopolo-La Fe, Avenida Fernando Abril Martorell, 106-Torre A, Planta 1ª, 46026, Valencia, Spain

    Rocio Rivera-Egea, Roberto González-Martín, Marcos Meseguer, Jose Remohí, Nicolas Garrido & Francisco Dominguez

  3. IVF Laboratory, IVIRMA Valencia, Plaza de la Policía Local, 3, 46015, Valencia, Spain

    Marcos Meseguer

  4. Reproductive Medicine Department, IVIRMA Valencia, Plaza de la Policía Local, 3, 46015, Valencia, Spain

    Jose Remohí

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  1. Rocio Rivera-Egea
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Contributions

RR designed the study, selected the patients, performed the analysis, and wrote the paper; NS helped in the design of the study and performed the protein profile analysis; RG helped in the statistical approach; MM collaborated in the protein profile studies and in the writing of the paper; JR recruited many of the patients analyzed and helped in the design of the paper; FD helped in the statistical approach and monitored, coordinated the sperm analysis and helped in the writing of the paper; NG designed the study, performed the statistical analysis, and helped in the writing of the paper.

Corresponding author

Correspondence to Rocio Rivera-Egea.

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Written informed consent was obtained from all patients and the study was approved by the Ethics Committee of IVI Valencia (#1209-C-108-RR).

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The authors declare no competing interests.

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Rivera-Egea, R., Sota, N., González-Martín, R. et al. Differential sperm proteomic profiles according to pregnancy achievement in intracytoplasmic sperm injection cycles: a pilot study. J Assist Reprod Genet 38, 1507–1521 (2021). https://doi.org/10.1007/s10815-021-02098-0

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