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High-resolution 1H-NMR spectroscopy indicates variations in metabolomics profile of follicular fluid from women with advanced maternal age

  • Assisted Reproduction Technologies
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Abstract

Aim

To reveal whether there are differences in follicular fluid metabolomics profile of women with advanced maternal age (AMA).

Method

The group with advanced maternal age includes 23 patients above the age of 40, and the control group includes 31 patients aged between 25 and 35 years and AMH values above 1.1 ng/mL with no low ovarian response history. A single follicular fluid sample from a MII oocyte obtained during the oocyte pick-up procedure was analyzed with high-resolution 1H-NMR (nuclear magnetic resonance) spectroscopy. The results were evaluated using advanced bioinformatics analysis methods.

Results

Statistical analysis of the NMR spectroscopy data from two groups showed that α-glucose and β-glucose levels of follicular fluid were decreased in the patients with AMA, while in contrast, lactate and trimethylamine N-oxide (TMAO) levels were increased in these patients compared with the controls. In addition to these, there was an increase in alanine levels and a decrease in acetoacetate levels in patients with AMA. However, these changes were not statistically significant.

Conclusion

Obtained results suggest that the follicular cell metabolism of patients with AMA is different from controls. These environmental changes could be associated with the low success rates of IVF treatment seen in these patients.

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Funding

This study was supported by the scientific research projects unit of Inonu University (Grant Number 2016/56).

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Authors and Affiliations

  1. Reproductive Sciences & Advanced Bioinformatics Application & Research Center, Inonu University, Malatya, Turkey

    B. Dogan, A. Karaer, G. Tuncay, N. Tecellioglu & A. Mumcu

  2. Department of Biomedical Engineering, School of Engineering, Inonu University, Malatya, Turkey

    B. Dogan

  3. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Inonu University, School of Medicine, Malatya, Turkey

    A. Karaer, G. Tuncay & N. Tecellioglu

  4. Laboratory of NMR, Scientific and Technological Research Center, Inonu University, Malatya, Turkey

    A. Mumcu

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  1. B. Dogan
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Correspondence to A. Karaer.

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All patients included in the study provided written informed consent, and the study protocol was permitted by the Clinical Research Ethics Committee (Number 2015/38).

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Dogan, B., Karaer, A., Tuncay, G. et al. High-resolution 1H-NMR spectroscopy indicates variations in metabolomics profile of follicular fluid from women with advanced maternal age. J Assist Reprod Genet 37, 321–330 (2020). https://doi.org/10.1007/s10815-020-01693-x

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  • DOI: https://doi.org/10.1007/s10815-020-01693-x

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