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Functional single nucleotide polymorphisms of matrix metalloproteinase 7 and 12 genes in idiopathic recurrent spontaneous abortion

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The aim of this study was to investigate the potential association of matrix metalloproteinase 7 (MMP7) -181 A/G and MMP12 -82 A/G functional single nucleotide polymorphisms (SNP) with idiopathic recurrent spontaneous abortion (IRSA) in Slovenian reproductive couples.

Methods

A case–control study was conducted on 149 couples with 3 or more consecutive idiopathic spontaneous pregnancy loses and 149 women and men with at least 2 live births and no history of pregnancy complications. Genotyping of MMP7 -181 A/G and MMP12 -82 A/G SNPs was performed using polymerase chain reaction and restriction fragment length polymorphism methods.

Results

There were no statistically significant differences in the distribution of MMP7 -181 A/G and MMP12 -82 A/G genotype, allele, or haplotype frequencies between IRSA patients and controls, as well as patients’ primary and secondary IRSA. We also found no association of MMP7 -181 A/G and MMP12 -82 A/G genotypes, alleles, and haplotypes with IRSA.

Conclusions

We found no evidence to support the association between IRSA and MMP7 -181 A/G and MMP12 -82 A/G SNPs in Slovenian reproductive couples.

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Acknowledgements

This study was supported by research grants “Genetic factors in the etiology of idiopathic recurrent spontaneous abortion” (University of Rijeka, Croatia, number 13.06.1.3.32) and “Gynecology and Reproduction: Genomics and Stem Cells” (Slovenia, number P3―0326).

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Authors and Affiliations

  1. Department of Biology and Medical Genetics, Faculty of Medicine, University of Rijeka, B. Branchetta 20, 51000, Rijeka, Croatia

    Anita Barišić, Nina Pereza, Miljenko Kapović & Saša Ostojić

  2. Clinical Institute of Medical Genetics, Department of Gynaecology and Obstetrics, UMC Ljubljana, 1000, Ljubljana, Slovenia

    Alenka Hodžić & Borut Peterlin

Authors
  1. Anita Barišić
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  2. Nina Pereza
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  3. Alenka Hodžić
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  4. Miljenko Kapović
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  5. Borut Peterlin
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  6. Saša Ostojić
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Corresponding author

Correspondence to Saša Ostojić.

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Ethical approval

All procedures involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Conflict of interest

The authors declare that they have no conflicts of interest.

Informed consent

Written informed consent was obtained from all individual participants included in the study. The study was approved by Slovenian and Croatian National Ethics’ Committees and was performed in accordance with the ethical standards as described in the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

Studies with animals

This article does not contain any studies with animals performed by any of the authors.

Additional information

Capsule We found no evidence to support the association between IRSA and MMP7 -181 A/G and MMP12 -82 A/G SNPs in Slovenian reproductive couples.

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Barišić, A., Pereza, N., Hodžić, A. et al. Functional single nucleotide polymorphisms of matrix metalloproteinase 7 and 12 genes in idiopathic recurrent spontaneous abortion. J Assist Reprod Genet 34, 365–371 (2017). https://doi.org/10.1007/s10815-016-0848-4

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  • DOI: https://doi.org/10.1007/s10815-016-0848-4

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