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Design of a Fluorescence-Enhanced Aptasensor for Sensitive Detection of Silver Ions

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Journal of Applied Spectroscopy Aims and scope

A highly sensitive fluorescence-enhanced aptasensor was designed to detect silver ions (Ag+) using metal-enhanced fluorescence. Interaction of Ag+ with cytosine nucleobases was used to achieve a low detection limit. Aptamer-modified gold nanoparticles (Au NPs) were mixed with 6-carboxyfluorescein (FAM)-labeled DNA to prepare the sensor. In a solution without Ag+, aptamer and FAM-labeled DNA strands remained free because of repulsion between cytosine. In the presence of Ag+, pairs of aptamer and FAM-labeled DNA strands formed double helices through cytosine–Ag+– cytosine interactions. These interactions brought FAM close to the Au NPs. The number of adenines repeats in the aptamer was altered to adjust the distance between the Au NPs and FAM, and provide controllable localized surface plasmon resonance. With the optimum number of adenine repeats (n = 24), the linear range for detection of Ag+ was 0.694 to 6.94 nmol/L and the detection limit was 0.694 nmol/L. The aptasensor showed excellent specificity and gave a strong detection signal for Ag+ present at trace concentrations to overcome issues associated with the detection of weak signals.

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References

  1. Y. C. Wu, K. Jiang, S. H. Luo, L. Cao, and H. Q. Wu, Spectrochim. Acta A, 206, 632–641 (2019).

  2. Y. G. Ko, W. S. Na, N. Singh, and D. O. Jang, J. Fluoresc., 29, No. 4, 945–952 (2019).

  3. Q. Long, Y. Q. Wen, H. T. Li, Y. Y. Zhang, and S. Z. Yao, J. Fluoresc., 27, No. 1, 205–211 (2017).

  4. Y. Lu, L. J. Meng, Y. Gao, D. L. Liao, and Y. X. Li, Anal. Biochem., 549, 21–25 (2018).

  5. Y. W. Zhang, A. Y. Ye, Y. W. Yao, and C. Yao, Sensors (Basel), 19, No. 2, 247–249 (2019).

  6. J. W. Qi, Z. Q. Chen, J. Chen, Y. D. Li, and W. Qiang, Opt. Express., 22, No. 12, 14688–14695 (2014).

  7. Y. F. Zhu, Y. S. Wang, B. Zhou, Y. Q. Huang, and X. J. Li, Spectrochim. Acta A, 189, 190–194 (2018).

  8. Z. Y. Chu, W. N. Wang, C. Y. Zhang, J. Ruan, and B. J. Chen, Chem. Eng. J., 375, Article ID 121927 (2019).

  9. L. C. Shi, J. S. Hu, X. F. Wu, S. P. Zhan, S. G. Hu, and Z. G. Tang, Dalton. T., 47, No. 46, 16445–16452 (2018).

  10. D. D. Tan, Y. He, X. J. Xing, Y. Zhao, H. W. Tang, and D. W. Pang, Talanta, 113, 26–30 (2013).

  11. T. H. Nguyen, S. P. Wren, T. Sun, and K. T. V. Grattan, TIEEE Sens. J., 20, 480–482 (2016).

  12. N. D. Acha, C. Elosua, J. M. Corres, and F. J. Arregui, Sensors (Basel), 19, 599, 1–34 (2019).

  13. J. Ding, H. Y. Li, C. Wang, J. Yang, Y. J. Xie, ACS Appl. Mater. Inter., 7, No. 21, 11369–11376 (2015).

  14. W. Xu, C. L. Ren, C. L. Teoh, J. J. Peng, and S. H. Gadre, Anal. Chem., 86, No. 17, 8763–8769 (2014).

  15. Z. Jiao, P. F. Zhang, H. W. Chen, C. Li, and L. Chen, Sens. Actuators B Chem., 295, 110–116 (2019).

  16. R. R. Gaddam, D. Vasudevan, R. Narayan, and K. V. Raju, RSC Adv., 100, No. 4, 57137–57143 (2014).

  17. F. Yarur, J. R. Macairan, and R. Naccache, Environ. Sci-Nano., 6, No. 4, 1121–1130 (2019).

  18. F. Firdaus, A. Farhi, M. Faraz, and M. Shakir, J. Lumin., 199, 475–482 (2018).

  19. Z. P. Zhou, H. D. Huang, Y. Chen, F. Liu, and C. Z. Huang, Biosens. Bioelectron., 52, 367–373 (2014).

  20. H. B. Teh, H. N. Wu, X. B. Zuo, and S. F. Y. Li, Sens. Actuators B Chem., 195, 623–629 (2014).

  21. N. Sui, L. Wang, T. F. Yan, F. Y. Liu, and J. Sui, Sens. Actuators B Chem., 202, 1148–1153 (2014).

  22. J. F. Lodeiro, C. Nunez, A. F. Lodeiro, E. Oliveira, and C. Lodeiro, Nanopart. Res., 16, No. 3, 1–12 (2014).

  23. J. J. Peng, J. Y. Li, Nunez, W. Xu, L. Wang, and D. D. Su, Anal. Chem., 90, No. 3, 1628–1634 (2018).

  24. G. Aragay, G. Alarcon, J. Pons, and A. Merkoci, J. Phys. Chem. C, 116, No. 2, 1987–1994 (2012).

  25. W. W. Qin, W. Dou, V. Leen, W. Dehaen, M. V. Auweraer, and N. Boens, RSC Adv., 6, No. 10, 7806–7816 (2016).

  26. S. S. Bayram, P. Green, and A. S. Blum, Spectrochim. Acta A, 195, 21–24 (2018).

  27. R. R. Kayumova, S. A. Peshkov, S. S. Ostakhov, and S. L. Khursan, High. Energ. Chem., 51, No. 1, 75–77 (2017).

  28. Y. Miyake, H. Togashi, M. Tashiro, and S. L. Khursan, J. Am. Chem. Soc., 128, No. 7, 2172–2173 (2006).

  29. W. H. Zhou, R. Saran, and J. Liu, Chem. Rev., 117, No. 12, 8272–8325 (2017).

  30. X. Wei, H. Li, Z. H. Li, M. Vuki, and Y. Fan, Anal. Bioanal. Chem., 402, No. 3, 1057–1063 (2012).

  31. C. W. Liu, C. C. Huang, and H. T. Chang, Langmuir., 24, No. 15, 8346–8350 (2008).

  32. Y. F. Pang, Z. Rong, R. Xiao, and S. Q. Wang, Sci. Rep. UK, 5, No. 1, 1–8 (2015).

  33. G. K. Wang, C. W. Shao, C. L. Yan, D. Li, and Y. F. Liu, J. Lumin., 210, 21–27(2019).

  34. L. D. Lavis, T. J. Rutkoski, and R. T. Raines, Sci. Anal. Chem., 79, No. 17, 6775–6782 (2007).

  35. J. C. Jin, B. B. Wang, Z. Q. Xu, X. H. He, H. F. Zou, Q. Q. Yang, F. L. Jiang, and Y. Liu, Sens. Actuators B Chem., 267, 627–635 (2018).

  36. B. Azizi, K. Farhadi, and N. Samadi, J. Anal. Chem., 75, No. 12, 1546–1553 (2020).

  37. G. P. Yan, Y. H. Wang, X. X. He, K. M. Wang, J. Su, Z. F. Chen, and Z. H. Qing, Talanta, 94, 178–183 (2012).

  38. Y. H. Lin and W. L. Teng, Chem. Commun., 43, 6619–6621 (2009).

  39. X. H. Gao, Y. Z. Lu, R. Z. Zhang, S. J. He, J. Ju, M. M. Liu, L. Li, and W. Chen, J. Mater. Chem. C, 3, No. 10, 2302–2309 (2015).

  40. J. Lee, J. Park, H. H. Lee, H. Park, H. I. Kim, and W. J. Kim, Biosens. Bioelectron., 68, 642–647(2015).

  41. Y. Yang, T. Liu, L. Cheng, G. S. Song, Z. Liu, and M. W. Chen, ACS Appl. Mater. Interfaces, 7, No. 14, 7526–7533 (2015).

  42. H. Li, S. Ye, J. Q. Guo, H. B. Wang, W. Yan, J. Song, and J. Qu, Nano Res., 12, 3075–3084 (2019).

  43. J. Q. Guo, S. Ye, H. Li, J. Song, and J. Qu, Dyes. Pigment, 183, Article ID 108723 (2020).

  44. J. Wang, A. Y. Liu, B. C. Wu, Q. L. Wen, Z. F. Pu, R. X. Zhao, J. Ling, and Q. Cao, Anal. Methods, 13, No. 18, 2099–2106 (2021).

  45. Z. F. Pu, J. Peng, Q. L. Wen, Y. Li, J. Ling, P. Liu, and Q. E. Cao, Dyes Pigment, 193, Article ID 109533 (2021).

  46. T. Khantaw, C. Boonmee, T. Tuntulani, and W. Ngeontae, Talanta, 115, 849–856 (2013).

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Authors and Affiliations

  1. School of Electrical and Information Engineering at Zhengzhou University of Light Industry, Zhengzhou, China

    L. Ren, G. Chen, Zh. Peng, X. Xu, P. Zhang, Z. Qin, Q. Chen, Y. Yan & L. Jiang

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  1. L. Ren
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  2. G. Chen
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  3. Zh. Peng
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  4. X. Xu
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  5. P. Zhang
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  6. Z. Qin
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  7. Q. Chen
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  8. Y. Yan
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  9. L. Jiang
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Correspondence to L. Jiang.

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Abstract of article is published in Zhurnal Prikladnoi Spektroskopii, Vol. 89, No. 5, p. 745, September–October, 2022.

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Ren, L., Chen, G., Peng, Z. et al. Design of a Fluorescence-Enhanced Aptasensor for Sensitive Detection of Silver Ions. J Appl Spectrosc 89, 984–991 (2022). https://doi.org/10.1007/s10812-022-01457-8

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