This study combined Raman technology and nanotechnology to detect periodontal inflammatory factors. We synthesized silver nanoparticles and made modifications to their surface to produce Ag NP-antibody probes. A sandwich immunoassay was developed for the sensitive detection of TNF-α and IL-1β. SERS intensity showed a good linear relationship with TNF-α and IL-1β concentrations in our study. The results of detection of these biomarkers in GCF indicate excellent specificity, high sensitivity, and great reproducibility of this immunoassay. Our immunoassay based on the SERS technique would facilitate clinical diagnoses of periodontal diseases.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
B. L. Pihlstrom, B. S. Michalowicz, and N. W. Johnson, Lancet, 366, 1809–1820 (2005).
G. C. Armitage, Periodontol. 2000, 34, 109–119 (2004).
H. Ito, Y. Numabe, S. Hashimoto, S. Sekino, E. Murakashi, and H. Ishiguro, J. Periodontol., 87, 1314–1319 (2016).
Q. Zhang, B. Chen, D. Zhu, and F. Yan, Arch. Oral. Biol., 72, 92–98 (2016).
O. Cakmak, Z. Tasdemir, C. A. Aral, S. Dundar, and H. B. Koca, J. Clin. Periodontol., 43, 1024–1031 (2016).
J. Liukkonen, U. K. Gürsoy, P. J. Pussinen, A. L. Suominen, and E. Könönen, J. Periodontol., 87, 1484–1491 (2016).
M. Kuboniwa, A. Sakanaka, E. Hashino, T. Bamba, E. Fukusaki, and A. Amano, J. Dent. Res., 95, 1381–1386 (2016).
R. C. Page, J. Periodontol., 63, 356–366 (1992).
J. Gamonal, A. Acevedo, A. Bascones, and O. Jorge, J. Periodontol., 71, 1535–1545 (2000).
N. R. Isola, D. L. Stokes, and T. Vo-Dinh, Anal. Chem., 70, 1352 (1998).
W. Ren, Y. Fang, and E. Wang, ACS Nano, 5, 6425–6433 (2011).
M. Y. Sha, H. Xu, M. J. Natan, and R. Cromer, J. Am. Chem. Soc., 130, 17214–17215 (2008).
Y. C. Cao, R. Jin, and C. A. Mirkin, Science, 297, 1536 (2002).
Y. Chen, H. Cheng, K. Tram, S. Zhang, Y. Zhao, L. Han, Z. Chen, and S. Huan, Analyst, 138, 2624–26231 (2013).
L. Wu, Z. Wang, S. Zong, H. Chen, C. Wang, S. Xu, and Y. Cui, Analyst, 138, 3450–3456 (2013).
E. Papadopoulou and S. E. Bell, Chemistry, 18, 5394 (2012).
G. F. Wang, R. J. Lipert, M. Jain, S. Kaur, S. Chakraboty, M. P. Torres, S. K. Batra, R. E. Brand, and M. D. Porter, Anal. Chem., 83, 2554–2561 (2011).
H. Hwang, H. Chon, J. Choo, and J. K. Park, Anal. Chem., 82, 7603–7610 (2010).
V. L. Schmit, R. Martoglio, and K. T. Carron, Anal. Chem., 84, 4233–4236 (2012).
P. V. Lee and D. Meisel, J. Phys. Chem., 86, 3391–3395 (1982).
C. Jehn, B. Kustner, P. Adam, A. Marx, P. Strobel, C. Schmuck, and S. Schlucker, Phys. Chem. Chem. Phys., 11, 7499–7504 (2009).
E. Papathanasiou, F. Teles, T. Griffin, E. Arguello, M. Finkelman, and J. Hanley, J. Periodont. Res., 49, 55–61 (2014).
Y. G. Sun, Y. D. Yin, B. T. Mayers, T. Herricks, and Y. N. Xia, Chem. Mater., 14, 4736–4745 (2002).
M. D. Porter, R. J. Lipert, L. M. Sioperko, G. Wang, and R. Narayanan, Chem. Soc. Rev., 37, 1001–1011 (2008).
Y. Wang, M. Salehi, M. Schütz, K. Rudi, and S. Schlücker, Analyst, 138, 1764–1771 (2013).
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Zhurnal Prikladnoi Spektroskopii, Vol. 86, No. 6, pp. 968–975, November–December, 2019.
Rights and permissions
About this article
Cite this article
Yang, H., Zhang, M.L., Yao, L.H. et al. Surface-Enhanced Raman Scattering-Based Immunoassay of Two Cytokines in Human Gingival Crevicular Fluid Of Periodontal Disease. J Appl Spectrosc 86, 1077–1083 (2020). https://doi.org/10.1007/s10812-020-00943-1
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10812-020-00943-1