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Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis

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Journal of Applied Spectroscopy Aims and scope

It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution–alternating least squares (MCR–ALS) was used to determine the ratio of the spectral components originating from phenol and proteins (Ph/Pr)fl , as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr)sp was calculated. The values of the (Ph/Pr)fl ratio and honey protein content after packaging depended on the quality of homogenization before packaging in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30, and 9, 3.14 for (Ph/Pr)fl and corresponding values 2.64, 2.18, and 12.75, 2.31 for (Ph/Pr)sp, in all pairs, the first value presenting the sample after extraction and the second value the sample after packaging in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased.

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Authors and Affiliations

  1. Institute for Multidisciplinary Research, University of Belgrade, P.O. Box 33, Belgrade, 11030, Serbia

    M. Stanković, D. Bartolić, B. Šikoparij, D. Spasojević, D. Mutavdžić & K. Radotić

  2. BioSensе Institute — Research Institute for Information Technologies in Biosystems, University of Novi Sad, Novi Sad, 21101, Serbia

    B. Šikoparij

  3. University of Belgrade, Faculty of Chemistry, P.O. Box 51, Belgrade, 11158, Serbia

    M. Natić

Authors
  1. M. Stanković
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  2. D. Bartolić
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  3. B. Šikoparij
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  4. D. Spasojević
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  5. D. Mutavdžić
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  6. M. Natić
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  7. K. Radotić
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Corresponding authors

Correspondence to M. Natić or K. Radotić.

Additional information

Published in Zhurnal Prikladnoi Spektroskopii, Vol. 86, No. 2, pp. 241–248, March–April, 2019.

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Stanković, M., Bartolić, D., Šikoparij, B. et al. Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis. J Appl Spectrosc 86, 256–263 (2019). https://doi.org/10.1007/s10812-019-00809-1

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  • DOI: https://doi.org/10.1007/s10812-019-00809-1

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