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Determination of Structural and Morphological Parameters of Human Bulbar Conjunctiva from Optical Diffuse Reflectance Spectra

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Journal of Applied Spectroscopy Aims and scope

We have developed a method for on-the-fl y retrieval of the volume concentration of blood vessels, the average diameter of the blood vessels, the blood oxygenation level, and the molar concentrations of chromophores in the bulbar conjunctiva from its diffuse reflectance spectra, measured when the radiation delivery and detection channels are spatially separated. The relationship between the diffuse reflectance spectrum of the conjunctiva and its unknown parameters is described in terms of an analytical model, constructed on the basis of a highly accurate approximation analog of the Monte Carlo method. We have studied the effect of localization of hemoglobin in erythrocytes and localization of erythrocytes in the blood vessels on the power of the retrieval of structural and morphological parameters for the conjunctiva. We developed a device for obtaining video images of the conjunctiva and contactless measurements of its diffuse reflectance spectrum. By comparing simulated diffuse reflectance spectra of the conjunctiva with the experimental measurements, we established a set of chromophores which must be taken into account in the model for reproducing the experimental data within the measurement error. We observed absorption bands for neuroglobin in the experimental spectra, and provided a theoretical basis for the possibility of determining its absolute concentrations in the conjunctiva. We have shown that our method can detect low bilirubin concentrations in blood.

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Authors and Affiliations

  1. Belorussian State University, 4 Nezavisimost′ Ave., Minsk, 220030, Belarus

    S. A. Lisenko, V. A. Firago & M. M. Kugeiko

  2. Belorussian State Medical University, Minsk, Belarus

    A. I. Kubarko

Authors
  1. S. A. Lisenko
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  2. V. A. Firago
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  3. M. M. Kugeiko
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  4. A. I. Kubarko
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Correspondence to S. A. Lisenko.

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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 83, No. 4, pp. 606–615, July–August, 2016.

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Lisenko, S.A., Firago, V.A., Kugeiko, M.M. et al. Determination of Structural and Morphological Parameters of Human Bulbar Conjunctiva from Optical Diffuse Reflectance Spectra. J Appl Spectrosc 83, 617–626 (2016). https://doi.org/10.1007/s10812-016-0337-7

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