A new natural inhibitor, ellagic acid (EA), was developed, and its inhibition efficiency on elastase was studied by spectroscopic methods. The experimental results proved that EA is a potent elastase inhibitor with an IC50 value of 1.44 mg/mL by UV-vis spectroscopy, and the inhibition mechanism of elastase was confirmed by fluorescence quenching. The interacting between EA and elastase was mainly based on the static quenching owing to the complex formation when the concentration of EA was ≤40 μM. Fluorescence quenching mainly occurred via dynamic quenching with increasing EA concentration. The thermodynamic parameters such as ΔH and ΔS were calculated to be –86.35 kJ/mol and –165.88 J/mol · K, respectively, indicating that the interactions between EA and elastase were mainly due to van der Waals forces or hydrogen bonding. The synchronous fl uorescence spectra showed that binding of EA to elastase can induce conformational changes in elastase.
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W. Meyer, K. Neurand, and B. Radke, Arch. Dermatol. Res., 270, 391–401 (1981).
W. F. Daamen, J. H. Veerkamp, J. C. M. Van Hest, and T. H. V. Kuppevelt, Biomaterials, 28, 4378–4398 (2007).
K. Ju Hee and A. K. R. Bandi. J. Med. Plants Res., 3, 914–920 (2009).
K. Morihara, Arch. Biochem. Biophys., 120, 68–78(1967).
J. Balo and I. Banga, Biochem. J., 46, 384–387(1950).
Y. C. Tsai, R. Y. Juang, S. F. Lin; S. W. Chen, M. Yamasaki, and G.Tamura, Appl. Environ. Microbiol., 54, 3156–3161 (1988).
T. D. Tetley, Thorax, 48, 560–565 (1993).
S. Umeki, Y. Niki, and R. Soejima, Am. J. Med. Sci., 296, 103–106 (1988).
K. C. Meyer, J. R. Lewandoski, J. J. Zimmerman, D. Nunley, W. J. Calhoun, and G. A. Dopico, Am. Rev. Respir. Dis., 144, 580–585(1991).
Y. H. Kim, K. S. Kim; C. S. Han, H. C. Yang, S. H. Park, K. I. Ko, S. H. Lee, K. H. Kim, N. H. Lee, J. M. Kim, and K. H. Son, Int. J. Cosmetic Sci., 29, 487–488 (2007).
P. A. Henriksen, M. Hitt, Z. Xing, J. Wang, C. Haslett, R. A. Riemersma, D. J. Webb, Y. V. Kotelevtsev, and J. M. Sallenave, J. Immunol., 172, 4535–4544 (2004).
G. M.Tremblay, E. Vachon, C. Larouche, and Y. Bourbonnais, Chest., 121, 582–588 (2002).
M. Zhang, Z. Zou, N. Maass, and R. Sager, Cancer Res., 55, 2537–2541 (1995).
M. L. Zani, S. M. Nobar, S. A. Lacour, S. Lemoine, C. Boudier, J. G. Bieth, and T. Moreau, Eur. J. Biochem., 271, 2370–2378 (2004).
N. Sultana and N. H. Lee. Phytother. Res., 21, 1171–1176 (2007).
H. K. Ju and A. K. R. Bandi, J. Med. Plants Res., 3, 914–920 (2009).
S. C. Barros, J. A. Martins, J. C. Marcos, and P. A. Cavaco, Enzyme Microb. Technol., 50, 107–114 (2012).
T. Hatano, Nat. Med., 49, 357–363 (1995).
T. Okuda, Yakugaku Zasshi, 115, 81–100 (1995).
Y. Y. Soong and P. J. Barlow. Food Chem., 97, 524–530 (2006).
N. Rangkadilok, S. Sitthimonchai, L. Worasuttayangkurn, C. Mahidol, M. Ruchirawat, and J. Satayavivad, Food Chem. Toxicol., 45, 328–336 (2007).
M. Yoshimura, Y. Watanabe, K. Kasai, J. Yamakoshi, and T. Koga. Biosci. Biotechnol. Biochem., 69, 2368–2373 (2005).
P. Zafrilla, F. Ferreres, and F. A. Tomás-Barberán, J. Agric. Food Chem., 49, 3651–3655 (2001).
H. Shimogaki, Y. Tanaka, H. Tamai, and M. Masuda, Int. J. Cosmetic Sci., 22, 291–304 (2000).
D. David and C. M. Thomas, J. Gen. Microbiol., 134, 43–52 (1988).
Y. Yumin, H. Qiuluan, F. Yanli, and S. Hongshuai, Spectrochim . Acta, A, 69, 432–436 (2008).
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Abstract of article is published in Zhurnal Prikladnoi Spektroskopii, Vol. 83, No. 1, p. 164, January–February, 2015
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Xing, X., Yang, X. & Cao, Y. Study of Ellagic Acid as a Natural Elastase Inhibitor by Spectroscopic Methods. J Appl Spectrosc 83, 149–155 (2016). https://doi.org/10.1007/s10812-016-0259-4
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DOI: https://doi.org/10.1007/s10812-016-0259-4