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Effect of Triplet T 1-State Population on Fluorescence Polarization Kinetics of Organic Fluorophores

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Journal of Applied Spectroscopy Aims and scope

The fluorescence polarization kinetics of fluorescent organic molecules and molecules with two interconverting photoisomeric forms and fixed molecular skeletons were investigated by numerical methods based on the solution of population balance equations. The depolarizing effect of the lowest triplet T 1 state and the polarization time dependence for quasi-continuous-wave (cw) and pulsed photoexcitation were studied using model compounds. The fluorescence depolarization with cw excitation was characterized by times that were shorter than the T 1-state lifetime; for the isomers, also by times that correlated with the photoisomerization times. Intense pulsed photoexcitation distorted significantly the initial oscillator orientation distribution in the singlet S 0 and S 1 states and could lead to sign reversal of the polarization.

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References

  1. G. Weber, in: Fluorescence and Phosphorescence Analysis, D. M. Hercules (Ed.), John Wiley & Sons, New York (1966), pp. 217–240.

  2. B. I. Stepanov and V. P. Gribkovskii, Introduction to Luminescence Theory [in Russian], Izd. Akad. Nauk BSSR, Minsk (1960), pp. 48–427.

    Google Scholar 

  3. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum Press, New York (1983) [Russian translation (1986), pp. 10–488].

  4. V. A. Gaisenok and A. M. Sarzheskii, Anisotropy of Absorption and Luminescence of Polyatomic Molecules [in Russian], Universitetskoe, Minsk (1986), pp. 5–123.

    Google Scholar 

  5. V. A. Kuz′mitskii, Khim. Fiz., 15, No. 12, 61–74 (1996).

    Google Scholar 

  6. M. Romagnoli, W. E. Moerner, F. M. Schellenberg, M. D. Levenson, and G. C. Bjorklund, J. Opt. Soc. Am. B, 1, No. 3, 341–348 (1984).

    Article  ADS  Google Scholar 

  7. S. M. Arabei, I. V. Stanishevsky, and K. N. Solovyov, Opt. Mem. Neural Networks, 10, No. 3, 157–164 (2001).

    Google Scholar 

  8. I. V. Stanishevskii and K. N. Solov′ev, Opt. Spektrosk., 96, No. 2, 255–263 (2004).

    Google Scholar 

  9. I. V. Stanishevskii, K. N. Solov′ev, S. M. Arabei, and V. A. Chernyavskii, Zh. Prikl. Spektrosk., 80, No. 3, 368–372 (2013) [I. V. Stanishevsky, K. N. Solovyov, S. M. Arabei, and V. A. Chernyavsky, J. Appl. Spectrosc., 80, 357–360 (2013)].

  10. C. Ringemann, A. Schonle, A. Giske, C. von Middendorff, S. W. Hell, and C. Eggeling, ChemPhysChem, 9, 612–624 (2008).

    Article  Google Scholar 

  11. I. V. Stanishevskii, K. N. Solov′ev, S. M. Arabei, and V. A. Chernyavskii, in: Proc. 2nd Int. Sci.-Pract. Conf. ″Processing and Quality Control of Agricultural Products″ [in Russian], March 26–27, 2015, Minsk, BGATU (2015), pp. 253–256.

  12. R. Avarmaa, Mol. Phys., 37, No. 2, 441–454 (1979).

    Article  ADS  Google Scholar 

  13. L. Benthem, R. B. M. Koehorst, and T. J. Schaafsma, J. Mol. Struct., 79, 455–460 (1982).

    Article  ADS  Google Scholar 

  14. K. Mauring, I. Renge, P. Sarv, and R. Avarmaa, Spectrochim. Acta, Part A, 43, No. 4, 507–514 (1987).

    Article  ADS  Google Scholar 

  15. I. V. Stanishevskii and K. N. Solov′ev, Zh. Prikl. Spektrosk., 65, No. 4, 532–538 (1998) [I. V. Stanishevskii and K. N. Solov′ev, J. Appl. Spectrosc., 65, 552–559 (1998)].

  16. I. V. Stanishevskii and K. N. Solov′ev, Opt. Spektrosk., 97, No. 2, 286–291 (2004).

    Google Scholar 

  17. G. A. Zagusta, V. N. Kotlo, S. F. Shkirman, and K. N. Solov′ev, Zh. Prikl. Spektrosk., 27, No. 1, 164–166 (1977) [G. A. Zagusta, V. N. Kotlo, S. F. Shkirman, and K. N. Solov′ev, J. Appl. Spectrosc., 27, 946–947 (1977)].

  18. B. Wehrle, H. H. Limbach, M. Kocher, O. Ermel, and E. Vogel, Angew. Chem., Int. Ed. Engl., 26, No. 9, 934–936 (1987).

    Article  Google Scholar 

  19. K. N. Solov′ev, L. L. Gladkov, A. S. Starukhin, and S. F. Shkirman, Spectroscopy of Porphyrins: Vibrational States [in Russian], Nauka i Tekhnika, Minsk (1985), pp. 12–415.

    Google Scholar 

  20. A. T. Gradyushko, A. N. Sevchenko, K. N. Solovyov, and M. P. Tsvirko, Photochem. Photobiol., 11, 387–400 (1970).

    Article  Google Scholar 

  21. V. A. Kuz′mitskii, K. N. Solov′ev, and M. P. Tsvirko, Porphyrins: Spectroscopy, Electrochemistry, Application [in Russian], Nauka, Moscow (1987), pp. 7–126.

    Google Scholar 

  22. A. S. Starukhin, I. V. Stanishevskii, and A. M. Shul′ga, Opt. Spektrosk., 59, No. 5, 1037–1040 (1985).

    Google Scholar 

  23. V. A. Kuz′mitskii and V. N. Kotlo, Zh. Prikl. Spektrosk., 21, No. 4, 636–643 (1974) [V. A. Kuz′mitskii and V. N. Kotlo, J. Appl. Spectrosc., 21, 1321–1326 (1974)].

  24. I. E. Zalesskii, V. N. Kotlo, K. N. Solov′ev, and S. F. Shkirman, Zh. Prikl. Spektrosk., 20, No. 6, 1010–1015 (1974) [I. E. Zalesskii, V. N. Kotlo, K. N. Solov′ev, and S. F. Shirkman, J. Appl. Spectrosc., 20, 761–764 (1974)].

  25. I. K. Shushkevich, V. N. Knyukshto, V. A. Kuz′mitskii, V. N. Kopranenkov, A. M. Vorotnikov, and K. N. Solov′ev, Zh. Prikl. Spektrosk., 57, No. 1–2, 92–99 (1992) [I. K. Shushkevich, V. N. Knyukshto, V. A. Kuz′mitskii, V. N. Kopranenkov, A. M. Vorotnikov, and K. N. Solov′ev, J. Appl. Spectrosc., 57, 601–607 (1992)].

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Authors and Affiliations

  1. Belarusian State Agrarian Technical University, 99 Nezavisimost′ Ave., Minsk, 220023, Belarus

    I. V. Stanishevsky & V. A. Chernyavskii

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  1. I. V. Stanishevsky
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  2. V. A. Chernyavskii
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Correspondence to I. V. Stanishevsky.

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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 82, No. 5, pp. 666–672, September–October, 2015.

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Stanishevsky, I.V., Chernyavskii, V.A. Effect of Triplet T 1-State Population on Fluorescence Polarization Kinetics of Organic Fluorophores. J Appl Spectrosc 82, 726–731 (2015). https://doi.org/10.1007/s10812-015-0171-3

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  • DOI: https://doi.org/10.1007/s10812-015-0171-3

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