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Assessment of Microcirculatory Hemoglobin Levels in Normal and Diabetic Subjects using Diffuse Reflectance Spectroscopy in the Visible Region — a Pilot Study

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Journal of Applied Spectroscopy Aims and scope

Light-based diagnostic techniques provide a minimally invasive way for selective biomarker estimation when tissues transform from a normal to a malignant state. Spectroscopic techniques based on diffuse reflectance characterize the changes in tissue hemoglobin/oxygenation levels during the tissue transformation process. Recent clinical investigations have shown that changes in tissue oxygenation and microcirculation are observed in diabetic subjects in the initial and progressive stages. In this pilot study, we discuss the potential of diffuse reflectance spectroscopy (DRS) in the visible (Vis) range to differentiate the skin microcirculatory hemoglobin levels between normal and advanced diabetic subjects with and without neuropathy. Average concentration of hemoglobin as well as hemoglobin oxygen saturation within the probed tissue volume is estimated for a total of four different sites in the foot sole. The results indicate a statistically significant decrease in average total hemoglobin and increase in hemoglobin oxygen saturation levels for diabetic foot compared with a normal foot. The present study demonstrates the ability of reflectance spectroscopy in the Vis range to determine and differentiate the changes in tissue hemoglobin and hemoglobin oxygen saturation levels in normal and diabetic subjects.

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Authors and Affiliations

  1. Biophotonics Lab, Biomedical Engineering Group, Department of Applied Mechanics, Indian Institute of Technology Madras, Chennai, 600 036, India

    N. Sujatha, B. S. Suresh Anand & K. Bala Nivetha

  2. Diabetic Foot Clinic, Sundaram Medical Foundation, Chennai, 600 040, India

    V. B. Narayanamurthy, V. Seshadri & R. Poddar

Authors
  1. N. Sujatha
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  2. B. S. Suresh Anand
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  3. K. Bala Nivetha
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  4. V. B. Narayanamurthy
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  5. V. Seshadri
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  6. R. Poddar
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Correspondence to N. Sujatha.

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Published in Zhurnal Prikladnoi Spektroskopii, Vol. 82, No. 3, pp. 423–428, May–June, 2015.

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Sujatha, N., Anand, B.S.S., Nivetha, K.B. et al. Assessment of Microcirculatory Hemoglobin Levels in Normal and Diabetic Subjects using Diffuse Reflectance Spectroscopy in the Visible Region — a Pilot Study. J Appl Spectrosc 82, 432–437 (2015). https://doi.org/10.1007/s10812-015-0125-9

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  • DOI: https://doi.org/10.1007/s10812-015-0125-9

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