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PAR regulation of photoprotection in Phaeodactylum tricornutum (Bacillariophyceae): Roles of doses and irradiances

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Abstract

Photosynthetic diatoms are exposed to a rapid and unexpected variations in light intensity that can modulate the contents of photosynthetic pigments and the carotenoid profile. In this study, the influence of light availability on photoprotection in the diatom Phaeodactylum tricornutum was investigated. Experimental cultures were exposed to three irradiances (100, 200 and 400 µmol photons m−2 s−1) and two light: dark cycles (12:12 and 24:00 h) that resulted in different light energy daily doses (D = 4.32 mol photons m−2), where the treatments were named in NDxh (N = amount of daily doses D, and x = photoperiod in hours). The samples treated at different doses were compared in terms of cell growth, chlorophyll-a fluorescence, pigment content and gene transcription levels. Specific growth rate was 1.9-fold higher in 8D24h daily dose (34.56 mol photons m−2) with 3 times higher light absorption in comparison to the lowest light energy dose. Also, at higher light intensities the content of chlorophyll-a, fucoxanthin and diadinoxanthin in P. tricornutum was lower, while the regulation of the xanthophyll cycle was achieved by the highest light energy doses. The transcriptional profiles of ZEP1, ZEP2, VDL1 and VDL2 genes were influenced by the highest light energy doses, on the other hand VDE and ZEP3 genes were poorly regulated by light. In addition, a similar transcription pattern was found for two isoforms of ZEP genes as well as in VDL genes. This study demonstrated that light energy doses and irradiances affect the photoacclimation and photoprotection responses.

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Acknowledgements

The authors would like to thank the Brazilian Ministry of Science, Technology, Innovation and Communications (MCTIC) for financial support provided.

Funding

The research was funded by the Funding Authority for Studies and Projects (FINEP) (Agreement No. 01.10.0457.00) and by the National Council for Scientific and Technological Development (CNPq) (Case No. 407513/2013–2).

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Authors and Affiliations

  1. Laboratory of Algae Cultivation, Department of Aquaculture, Center of Agrarian Sciences, Federal University of Santa Catarina, Beco Dos Coroas, Florianópolis, , Santa Catarina, 503, 88061-600, Brazil

    Herculano Cella, Camila Nader, Rafael Garcia Lopes & Roberto Bianchini Derner

  2. Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina, Florianopolis, Brazil

    Camila Lisarb Velasquez Bastolla, Jacó Joaquim Mattos, Afonso Celso Dias Bainy & Maria Risoleta Freire Marques

  3. Laboratory of Phycology, Department of Botany, Center of Biological Sciences, Federal University of Santa Catarina, Florianopolis, Brazil

    José Bonomi-Barufi, Carlos Yure B. Oliveira & Leonardo Rubi Rörig

  4. Laboratory of Plant Morphogenesis and Biochemistry, Center of Agrarian Sciences, Federal University of Santa Catarina, Florianopolis, Brazil

    Cláudia Marlene Bauer & Marcelo Maraschin

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  1. Herculano Cella
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  2. Camila Nader
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  3. Camila Lisarb Velasquez Bastolla
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Contributions

All authors participated in the conception and design of the experiment. HC, CN, JBB and LRR performed the fluorescence analysis. HC, CLVB and JJM performed the molecular analysis. HC, CMB and MM performed the pigment analysis. HC, JBB and CYBO interpreted the data and discussed the results. HC drafted the article. RGL, JBB, MM, LRR, ACDB, MRFM and RBD critically reviewed the article and contributed with intellectual input. All authors approved submission of the article.

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Correspondence to Herculano Cella.

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Cella, H., Nader, C., Bastolla, C.L.V. et al. PAR regulation of photoprotection in Phaeodactylum tricornutum (Bacillariophyceae): Roles of doses and irradiances. J Appl Phycol 35, 2177–2191 (2023). https://doi.org/10.1007/s10811-023-03042-8

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