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Antibacterial activity of Caulerpa racemosa against pathogenic bacteria promoting “ice-ice” disease in the red alga Gracilaria verrucosa

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Abstract

Increasing ocean temperatures associated with climate change have triggered the occurrence of diseases in marine resources such as macroalgae or seaweed. “Ice-ice” is one of the most devastating diseases affecting economically important seaweeds such as Gracilaria and Eucheuma. In this study, we investigate the bacterial composition of diseased and healthy Gracilaria verrucosa, a red seaweed cultured in brackish water ponds in Takalar, Indonesia. Morphologic and phenotypic characteristics showed that the isolates from diseased Gracilaria belong to various genera: Vibrio, Chromobacterium, Flavobacterium, Pseudomonas, and Achromobacter. Several bacteria were also isolated from healthy Gracilaria including Corynebacterium, Serratia, Shigella, Micrococcus, Proteus, and Flavobacterium. Using Koch’s postulates, bacterial pathogenicity was established by bath exposure of naïve G. verrucosa to each of the bacteria isolated from diseased Gracilaria resulting in symptom characteristic of “ice-ice” disease. The antibacterial property of the green seaweed Caulerpa racemosa against the pathogenic bacteria was assessed using extracts that were prepared with solvents of various polarities such as hexane, chloroform, ethyl acetate, methanol, and methanol-water. The highest antibacterial activity was observed in methanol extracts Caulerpa while extracts using the other solvents showed moderate to low activities. These findings demonstrate the potential of Caulerpa to inactivate bacterial pathogens associated with “ice-ice” disease.

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Funding

This study received support from the Ministry of Research, Technology and Higher Education of the Republic of Indonesia through the World Class Professor Program Scheme A (N0.168.A9/D2/KP/2017).

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Authors and Affiliations

  1. Faculty of Marine Science and Fisheries, Hasanuddin University, Jl. Perintis Kemerdekaan, Km 10, Tamalanrea, Makassar, 90245, Indonesia

    Elmi Nurhaidah Zainuddin, Hilal Anshary, Huyyirnah Huyyirnah & Ridha Hiola

  2. Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA

    Dolores V. Baxa

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  1. Elmi Nurhaidah Zainuddin
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Zainuddin, E.N., Anshary, H., Huyyirnah, H. et al. Antibacterial activity of Caulerpa racemosa against pathogenic bacteria promoting “ice-ice” disease in the red alga Gracilaria verrucosa. J Appl Phycol 31, 3201–3212 (2019). https://doi.org/10.1007/s10811-019-01805-w

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