Skip to main content
SpringerLink
Log in

Increased lipid productivity of Acutodesmus dimorphus using optimized pulsed electric field

  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

An alternative stress-inducing method, a low-energy pulsed electric field (LE-PEF), was used to improve the lipid productivity of microalgae cultures. A large shift in the Nile-Red-stained peaks toward a higher intensity in fluorescent-activated cell sorting, suggestive of an increased neutral lipid content, was observed when 10 kV LE-PEF pulses were applied to 800 mL batch cultures of Acutodesmus dimorphus. The optimal LE-PEF on-off cycle treatment for A. dimorphus was 2 s on–60 s off for 15 min with 6 cycles per day. Under these optimal LE-PEF treatment conditions, A. dimorphus showed an overall 28.8 % increase in lipid productivity. Therefore, based on these results, LE-PEF can be regarded as a promising tool to avoid the lipid content and growth rate trade-off and improve the lipid productivity of microalgae in various cultivation systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Amiali M, Ngadi M, Smith J, Raghavan G (2007) Synergistic effect of temperature and pulsed electric field on inactivation of Escherichia coli O157: H7 and Salmonella enteritidis in liquid egg yolk. J Food Eng 79:689–694

    Article  Google Scholar 

  • Antezana Zbinden MD, Sturm BSM, Nord RD, Carey WJ, Moore D, Shinogle H, Stagg-Williams SM (2013) Pulsed electric field (PEF) as an intensification pretreatment for greener solvent lipid extraction from microalgae. Biotechnol Bioeng 110:1605–1615

    Article  CAS  Google Scholar 

  • Beebe SJ, Fox PM, Rec LJ, Somers K, Stark RH, Schoenbach KH (2002) Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: apoptosis induction and tumor growth inhibition. IEEE Trans Plasma Sci 30:286–292

    Article  CAS  Google Scholar 

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  PubMed  Google Scholar 

  • Choi GG, Kim BH, Ahn CY, Oh HM (2011) Effect of nitrogen limitation on oleic acid biosynthesis in Botryococcus braunii. J Appl Phycol 23:1031–1037

    Article  CAS  Google Scholar 

  • Couée I, Sulmon C, Gouesbet G, El Amrani A (2006) Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. J Exp Bot 57:449–459

    Article  PubMed  Google Scholar 

  • Donsì F, Ferrari G, Pataro G (2010) Applications of pulsed electric field treatments for the enhancement of mass transfer from vegetable tissue. Food Eng Rev 2:109–130

    Article  Google Scholar 

  • Dunn J (2001) Pulsed electric field processing: an overview. In: Barbosa-Cánovas GV, Howard Zhang Q (eds) Pulsed electric fields in food processing: fundamental aspects and applications. Technomic Publishing, Lancaster, pp 1–30

    Google Scholar 

  • Griffiths MJ, Harrison ST (2009) Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 21:493–507

    Article  CAS  Google Scholar 

  • Guschina IA, Harwood JL (2006) Lipids and lipid metabolism in eukaryotic algae. Prog Lipid Res 45:160–186

    Article  CAS  PubMed  Google Scholar 

  • Hanelt D, Melchersmann B, Wiencke C, Nultsch W (1997) Effects of high light stress on photosynthesis of polar macroalgae in relation to depth distribution. Mar Ecol Prog Ser 149:255–266

    Article  CAS  Google Scholar 

  • Hessen DO, Van Donk E (1993) Morphological changes in Scenedesmus induced by substances released from Daphnia. Arch Hydrobiol 127:129–140

    Google Scholar 

  • Hillebrand H, Dürselen CD, Kirschtel D, Pollingher U, Zohary T (1999) Biovolume calculation for pelagic and benthic microalgae. J Phycol 35:403–424

    Article  Google Scholar 

  • Khozin-Goldberg I, Shrestha P, Cohen Z (2005) Mobilization of arachidonyl moieties from triacylglycerol into chloroplastic lipids following recovery from nitrogen starvation of the microalga Parietochloris incise. Biochim Biophys Acta Mol Cell Biol Lipids 1738:63–71

    Article  CAS  Google Scholar 

  • Kopplow O, Barjenbruch M, Heinz V (2004) Sludge pre-treatment with pulsed electric fields. Water Sci Technol 49:123–129

    CAS  PubMed  Google Scholar 

  • Lv JM, Cheng LH, Xu XH, Zhang L, Chen HL (2010) Enhanced lipid production of Chlorella vulgaris by adjustment of cultivation conditions. Bioresour Technol 101:6797–6804

    Article  CAS  PubMed  Google Scholar 

  • Mata TM, Martins AA, Caetano NS (2010) Microalgae for biodiesel production and other applications: a review. Renew Sustain Energy Rev 14:217–232

    Article  CAS  Google Scholar 

  • Menon KR, Balan R, Suraishkumar G (2013) Stress induced lipid production in Chlorella vulgaris: relationship with specific intracellular reactive species levels. Biotechnol Bioeng 110:1627–1636

    Article  CAS  PubMed  Google Scholar 

  • Min S, Evrendilek GA, Zhang HQ (2007) Pulsed electric fields: processing system, microbial and enzyme inhibition, and shelf life extension of foods. IEEE Trans Plasma Sci 35:59–73

    Article  CAS  Google Scholar 

  • Pancha I, Chokshi K, George B, Ghosh T, Paliwal C, Maurya R, Mishra S (2014) Nitrogen stress triggered biochemical and morphological changes in the microalgae Scenedesmus sp. CCNM 1077. Bioresour Technol 156:146–154

    Article  CAS  PubMed  Google Scholar 

  • Rhee GY, Gotham IJ (1981) The effect of environmental factors on phytoplankton growth: Temperature and the interactions of temperature with nutrient limitation1. Limnol Oceanogr 26:635–648

  • Sheng J, Vannela R, Rittmann BE (2011) Evaluation of cell-disruption effects of pulsed-electric-field treatment of Synechocystis PCC 6803. Environ Sci Technol 45:3795–3802

    Article  CAS  PubMed  Google Scholar 

  • Solovchenko AE (2012) Physiological role of neutral lipid accumulation in eukaryotic microalgae under stresses. Russ J Plant Physiol 59:167–176

    Article  CAS  Google Scholar 

  • Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35:171–205

    CAS  PubMed  PubMed Central  Google Scholar 

  • Tammam AA, Fakhry EM, El-Sheekh M (2011) Effect of salt stress on antioxidant system and the metabolism of the reactive oxygen species in Dunaliella salina and Dunaliella tertiolecta. Afr J Biotechnol 10:3795–3808

    CAS  Google Scholar 

  • Toepfl S, Mathys A, Heinz V, Knorr D (2006) Potential of high hydrostatic pressure and pulsed electric fields for energy efficient and environmentally friendly food processing. Food Rev Int 22:405–423

    Article  CAS  Google Scholar 

  • Yoo C, La HJ, Kim SC, Oh HM (2015) Simple processes for optimized growth and harvest of Ettlia sp. by pH control using CO2 and light irradiation. Biotechnol Bioeng 112:288–296

    Article  CAS  PubMed  Google Scholar 

  • Zimmermann U, Pilwat G, Riemann F (1974) Dielectric breakdown of cell membranes. Biophys J 14:881–899

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

This research was supported by grants from the Advanced Biomass R&D Center, a Global Frontier Program, funded by the Korean Ministry of Science, ICT and Future Planning and the KRIBB Research Initiative Program.

Author information

Authors and Affiliations

  1. Bioenergy and Biochemical Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea

    Hyun-Joon La, Seong-Hyun Seo, Ankita Srivastava, Jae-Yon Lee & Hee-Mock Oh

  2. Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701, Republic of Korea

    Gang-Guk Choi

  3. Electric Propulsion Research Center, Korea Electrotechnology Research Institute, Changwon, 642-120, Republic of Korea

    Chuhyun Cho & Yun-Sik Jin

  4. Bureau of Ecological Conservation Research, National Institute of Ecology, Seocheon, 325-813, Republic of Korea

    Beom-Ho Jo

Authors
  1. Hyun-Joon La
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gang-Guk Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chuhyun Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Seong-Hyun Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ankita Srivastava
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Beom-Ho Jo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jae-Yon Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yun-Sik Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Hee-Mock Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hee-Mock Oh.

Additional information

Hyun-Joon La and Gang-Guk Choi are the co-first authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

La, HJ., Choi, GG., Cho, C. et al. Increased lipid productivity of Acutodesmus dimorphus using optimized pulsed electric field. J Appl Phycol 28, 931–938 (2016). https://doi.org/10.1007/s10811-015-0674-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10811-015-0674-6

Keywords

Search

Navigation