Abstract
Purpose
Periorbital fat atrophy is a known side effect of topical prostaglandin analogs (PA). This side effect may have implications in the treatment of diseases like thyroid orbitopathy. In this in vivo study we aimed to evaluate the effects of retrobulbar injection of three different PAs on orbital fat.
Methods
Eighteen adult male Wistar-albino rats were divided into three groups of six animals. 0.1 ml of 0.03% bimatoprost, 0.005% latanoprost, or 0.005% travoprost was injected into the right orbits and saline was injected into the left orbits, as controls. Both orbits were exenterated after 3 weeks. Histological cross-sections were analyzed using ImageJ image analysis software. Intraconal adipocyte density was calculated.
Results
There was no significant difference in the adipocyte density between the PA injected orbits and the control side in each of the three groups. When calculations from all three groups were analyzed together, again the difference in the adipocyte density between the PA injected orbits and the control side was not significant.
Conclusion
No significant fat atrophy was noted in this rat model three weeks after retrobulbar injection of PAs. To evaluate retrobulbar injection of PA as a potential therapy for orbital diseases with fat proliferation, in vivo studies in different animal models, higher concentrations of PA, or longer follow-up duration are required.
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Acknowledgements
This research received support from Turkish Ophthalmological Association Ankara Regional Branch, Ankara, Turkey.
Funding
This research received support from Turkish Ophthalmological Association Ankara Regional Branch, Ankara, Turkey.
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YA conceived and designed the work, acquired data, interpreted the results, drafted and revised the manuscript. OI and SC did animal experiment, acquired data, analyzed the results and drafted the manuscript. PA and BK prepared and analyzed the histological cross-sections. OO and AA acquired data and revised the manuscript. JK performed the statistical analysis. YA, OI, SC, PA, BK, OO, AA and JK approved the final version and agreed to be accountable for all aspects of the work.
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None of the authors have no competing interests to declare that are relevant to the content of this article. There are no financial disclosures for any of the authors.
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Ethics approval was obtained from the Ethics Committee on Animal Experiments, Hacettepe University, Ankara, Turkey. All experimental procedures adhered to the Association for Research in Vision and Ophthalmology statement for the Use of Animals in Ophthalmic and Vision Research.
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Cosan, S., İnam, O., Kaya, B. et al. Effect of retrobulbar prostaglandin analog injection on orbital fat in rats. Int Ophthalmol 43, 4985–4990 (2023). https://doi.org/10.1007/s10792-023-02901-0
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DOI: https://doi.org/10.1007/s10792-023-02901-0