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Resveratrol ameliorates DSS-induced ulcerative colitis by acting on mouse gut microbiota

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Abstract

Objective and design

Ulcerative colitis (UC) is a multi-faceted, recurrent immune disorder caused by dextran sulfate sodium (DSS). The intestinal microbiota has multiple functions in the host, so UC requires long-term potent medication. The effect of resveratrol (RSV) has seldom been reported, and this study researched that. Herein, the effect of RSV and Grape seed oil that anti-inflammatory ability in experimental mice was explored, also why RSV altered Gut Microbiota has been researched.

Materials and methods

In this experiment, the effects of experimental drugs on colon length in mice with DSS-induced colitis were compared. H&E Staining was performed on serial sections of colon tissues and histological scores were determined for all groups. The expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α) in the colon tissue of mice was detected by immunohistochemical staining. In the end, the α-diversity index, sobs index, and rarefaction curve of the cecal and colon microbiota of different groups of mice were measured. Bray–Curtis-based Venn diagram of PCoA (principal coordinate analysis) and OTUs distribution in mouse gut microbiota were obtained.

Results

The results showed that the use of 40 mg/kg RSV (high dose) significantly reduced the severity of UC. The use of 10 mg/kg RSV (low dose) significantly reduced the effect of shortened colon length in DSS mice. Compared with the DSS-treated group, the levels of COX-2 and TNF-α in the colon tissues of RSV + DSS-treated mice were significantly decreased. According to this experiment, 19 mouse gut microbiota species had a relative abundance greater than 0.1%, with Beerella, Bacteroides, Helicobacter, Oscillator, and cecum pylori being more abundant in the colon than in the colon. A higher relative abundance of Lachnospira NK4A136 was observed in DSS and RSV groups compared with the control group, whereas the opposite was observed for Alloprevotella. This proves that resveratrol increases the uniformity and diversity of gut microbes to a certain extent, and has a protective effect on the gut.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Abbreviations

COX-2:

Cyclooxygenase-2

DSS:

Dextran sulfate sodium

GSO:

Grape seed oil

HE:

Hematoxylin–eosin

ME:

Meanwhile, Mesalazine

OTUs:

Operational Taxonomic Units

PCoA:

Principal coordinate analysis

RSV:

Resveratrol

TNF-α:

Tumor necrosis factor-α

UC:

Ulcerative colitis

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Acknowledgements

We thank all members of the laboratory for their technical support, academic discussions, and the funding of the Guangdong Education Department of China (Grant No. 2017KQNCX054) and the Special Fund of Guangdong Academy of Science (2018GDASCX-0808).

Funding

This study was financially supported by the Guangdong Education Department of China (Grant No. 2017KQNCX054) and the Special Fund of Guangdong Academy of Science (2018GDASCX-0808).

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Author notes

  1. Bing Yu and Ying Wang have contributed equally to this work.

Authors and Affiliations

  1. School of Materials and Energy, Guangdong University of Technology, Guangzhou, 510006, China

    Bing Yu

  2. Department of Traditional Chinese Medicine, Institute of Guangdong Geriatric, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510080, China

    Hui Li

  3. School of Biomedical and Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, Guangdong University of Technology, Guangzhou, 510006, China

    Ying Wang, Zekai Tan, Zhengyi Hong, Lewen Yao, Shiyuan Huang, Zexi Li, Lanyue Zhang & Hui Li

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Contributions

Investigation, data curation, writing—original draft, B.Y.; Investigation, validation, writing—review and editing, Y.W.; Investigation, Z.T.; Investigation, Z.H.; Formal analysis, L.Y.; Investigation, S.H.; Resources, Z.L.; Conceptualization, supervision, resources, funding acquisition, L.Z.; Conceptualization, methodology, supervision, writing—review and editing, H.L. All authors have read and agreed to the published version of the manuscript.

Corresponding authors

Correspondence to Lanyue Zhang or Hui Li.

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The authors declare that the research has no potential or perceived conflicts of interest.

Ethical approval

All animal experiments were performed following applicable institutional and governmental regulations concerning the ethical use of animals. The animal study was reviewed and approved by the Animal Ethics Committee of Guangdong University of Technology (approval documents: 20221015011).

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Yu, B., Wang, Y., Tan, Z. et al. Resveratrol ameliorates DSS-induced ulcerative colitis by acting on mouse gut microbiota. Inflammopharmacol (2024). https://doi.org/10.1007/s10787-024-01456-5

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