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Inhibitory Effects of Cold Atmospheric Plasma on Inflammation and Tumor-Like Feature of Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis

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Abstract

Rheumatoid arthritis (RA) is a chronic, debilitating systemic disease characterized by chronic inflammation and progressive joint destruction. Fibroblast-like synoviocytes (FLSs) are one of the most important players in the pathophysiology of RA, acting like tumor cells and secreting inflammatory cytokines. Previous research has shown that cold atmospheric plasma (CAP) inhibits cancer cells and may have anti-inflammatory properties. This study examined the effects of argon plasma jet-produced CAP on the suppression of invasion and inflammation caused by cultured RA-FLS. The findings revealed that CAP reduced cell viability and elevated the percentage of apoptotic RA-FLS by producing reactive oxygen species. Carboxyfluorescein diacetate succinimidyl ester (CFSE) staining confirmed that CAP could decrease the proliferation of RA-FLS. Furthermore, CAP effectively reduced the production of inflammatory factors (e.g., NF-κB and IL-6) as well as destructive factors like receptor activator of nuclear factor kappa-B ligand (RANKL) and matrix metalloproteinases-3 (MMP-3). These data suggest that CAP could be a promising treatment for slowing the progression of RA by reducing tumor-like features and inflammation in RA-FLS.

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AVAILABILITY OF DATA AND MATERIALS

The data and materials used in this study are available from the corresponding author on reasonable request.

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ACKNOWLEDGEMENTS

The authors thank the patients and their families for their support and cooperation. We want to acknowledge the staff of the departments associated with the care and management of the patients.

Funding

This study was financially supported by a grant (grant number: 6891) from the Research and Technology Council of Mazandaran University of Medical Sciences.

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Authors and Affiliations

  1. Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Science, Sari, Iran

    Fatemeh Faramarzi, Parisa Zafari & Alireza Rafiei

  2. Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran

    Mina Alimohammadi

  3. Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Science, Sari, Iran

    Monireh Golpour

  4. Mazandaran University of Medical Sciences, Orthopedic Research Center, Sari, Iran

    Salman Ghaffari

Authors
  1. Fatemeh Faramarzi
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  2. Parisa Zafari
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  3. Mina Alimohammadi
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  4. Monireh Golpour
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  5. Salman Ghaffari
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  6. Alireza Rafiei
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Contributions

All authors contributed to the study. FF: conceptualization, investigation, methodology, data curation, formal analysis, and writing–original draft; PZ: investigation, formal analysis, and methodology; MA: writing–review and editing; MG: investigation; SG: investigation and methodology; AR: funding acquisition, project administration, supervision, validation, and writing review and editing.

Corresponding author

Correspondence to Alireza Rafiei.

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Ethics Approval and Consent to Participate

The study was approved by the Ethics Committee of the Mazandaran University of Medical Sciences (ethical approval code: IR.MAZUMS.REC.1398.6891).

Consent for Publication

The manuscript is approved by all authors for publication.

Competing Interests

The authors declare no competing interests.

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Faramarzi, F., Zafari, P., Alimohammadi, M. et al. Inhibitory Effects of Cold Atmospheric Plasma on Inflammation and Tumor-Like Feature of Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis. Inflammation 45, 2433–2448 (2022). https://doi.org/10.1007/s10753-022-01703-3

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  • DOI: https://doi.org/10.1007/s10753-022-01703-3

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