Abstract
Lipopolysaccharide (LPS), known as lipoglycans and endotoxins found in the cell wall of some type of Gram-negative bacteria, causes acute lung inflammation (ALI). p-Coumaric acid (p-CA) possesses anti-inflammatory and anti-oxidative activities. The main purpose of our research was to explore the effect of p-CA on LPS-induced inflammation. In part I, 32 rats were divided into four groups: Control, LPS (5 mg/kg), p-CA (100 mg/kg), and LPS + p-CA to investigate acute lung inflammation caused by LPS. In part II, the effect of LPS-stimulated inflammatory response on A549 cells was investigated. The dosage of LPS and p-CA which used in this part was 1 μg/ml and 20 mM, respectively. ALI rats showed an elevation in antioxidant activity, TNF-alpha, IL-6, MDA, inflammatory parameters, and Nrf2 gene expression. Although pre-treatment with p-CA could return these changes approximately to normal condition in all two-part studies (in vivo and in vitro). The results of in vivo and in vitro study showed that LPS induced lung inflammation. Pre-treatment with p-CA causes modulating of oxidative stress in inflammatory condition in lung injury and A549 cell.
Similar content being viewed by others
References
Kang, P., K.Y. Kim, H.S. Lee, S.S. Min, and G.H. Seol. 2013. Anti-inflammatory effects of anethole in lipopolysaccharide-induced acute lung injury in mice. Life Sciences 93 (24): 955–961.
Zuyderduyn, S., M. Sukkar, A. Fust, S. Dhaliwal, and J. Burgess. 2008. Treating asthma means treating airway smooth muscle cells. The European Respiratory Journal 32 (2): 265–274.
Bellani, G., J.G. Laffey, T. Pham, E. Fan, L. Brochard, A. Esteban, L. Gattinoni, F. van Haren, A. Larsson, D. McAuley, M. Ranieri, G. Rubenfeld, B.T. Thompson, H. Wrigge, A.S. Slutsky, A. Pesenti, LUNG SAFE Investigators, and ESICM Trials Group. 2016. Epidemiology, patterns of care, and mortality for patients with acute respiratory distress syndrome in intensive care units in 50 countries. Jama. 315 (8): 788–800.
Brun-Buisson, C., C. Minelli, G. Bertolini, L. Brazzi, J. Pimentel, K. Lewandowski, J. Bion, J.A. Romand, J. Villar, A. Thorsteinsson, P. Damas, A. Armaganidis, F. Lemaire, and ALIVE Study Group. 2004. Epidemiology and outcome of acute lung injury in European intensive care units. Intensive Care Medicine 30 (1): 51–61.
Thimmulappa, R.K., H. Lee, T. Rangasamy, S.P. Reddy, M. Yamamoto, T.W. Kensler, et al. 2016. Nrf2 is a critical regulator of the innate immune response and survival during experimental sepsis. The Journal of Clinical Investigation 116 (4): 984–995.
Ishii, Y., K. Itoh, Y. Morishima, T. Kimura, T. Kiwamoto, T. IIzuka, A.E. Hegab, T. Hosoya, A. Nomura, T. Sakamoto, M. Yamamoto, and K. Sekizawa. 2005. Transcription factor Nrf2 plays a pivotal role in protection against elastase-induced pulmonary inflammation and emphysema. Journal of Immunology 175 (10): 6968–6975.
Osburn, W.O., B. Karim, P.M. Dolan, G. Liu, M. Yamamoto, D.L. Huso, and T.W. Kensler. 2007. Increased colonic inflammatory injury and formation of aberrant crypt foci in Nrf2-deficient mice upon dextran sulfate treatment. International Journal of Cancer 121 (9): 1883–1891.
Bayan, L., P.H. Koulivand, and A. Gorji. 2014. Garlic: a review of potential therapeutic effects. Avicenna J Phytomed 4 (1): 1–14.
Lodovici, M., S. Caldini, L. Morbidelli, V. Akpan, M. Ziche, and P. Dolara. 2009. Protective effect of 4-coumaric acid from UVB ray damage in the rabbit eye. Toxicol. 255 (1–2): 1–5.
Pei, K., J. Ou, J. Huang, and S. Ou. 2016. p-Coumaric acid and its conjugates: dietary sources, pharmacokinetic properties and biological activities. Journal of the Science of Food and Agriculture 96 (9): 2952–2962.
Vauzour, D., G. Corona, and J.P. Spencer. 2010. Caffeic acid, tyrosol and p-coumaric acid are potent inhibitors of 5-S-cysteinyl-dopamine induced neurotoxicity. Archives of Biochemistry and Biophysics 501 (1): 106–111.
Ekinci Akdemir, F.N., M. Albayrak, M. Çalik, Y. Bayir, and I. Gülçin. 2017. The protective effects of p-coumaric acid on acute liver and kidney damages induced by cisplatin. Biomed. 5 (2): E18.
Jiang, H., Y. Zhu, H. Xu, Y. Sun, and Q. Li. 2010. Activation of hypoxia-inducible factor-1α via nuclear factor-κB in rats with chronic obstructive pulmonary disease. Acta Biochimica et Biophysica Sinica 42 (7): 483–488.
Szabo, S., S.N. Ghosh, B.L. Fish, S. Bodiga, R. Tomic, G. Kumar, N.V. Morrow, J.E. Moulder, E.R. Jacobs, and M. Medhora. 2010. Cellular inflammatory infiltrate in pneumonitis induced by a single moderate dose of thoracic x radiation in rats. Radiation Research 173 (4): 545–556.
Dianat, M., M. Radan, M. Badavi, S.A. Mard, V. Bayati, and M. Ahmadizadeh. 2018. Crocin attenuates cigarette smoke-induced lung injury and cardiac dysfunction by anti-oxidative effects: the role of Nrf2 antioxidant system in preventing oxidative stress. Respiratory Research 19 (1): 58.
Kode, A., S. Rajendrasozhan, S. Caito, S.-R. Yang, I.L. Megson, and I. Rahman. 2008. Resveratrol induces glutathione synthesis by activation of Nrf2 and protects against cigarette smoke-mediated oxidative stress in human lung epithelial cells. American Journal of Physiology. Lung Cellular and Molecular Physiology 294 (3): L478–LL88.
Chu, X., X. Ci, J. He, L. Jiang, M. Wei, Q. Cao, M. Guan, X. Xie, X. Deng, and J. He. 2012. Effects of a natural prolyl oligopeptidase inhibitor, rosmarinic acid, on lipopolysaccharide-induced acute lung injury in mice. Molecules. 17 (3): 3586–3598.
Kaulmann, A., and T. Bohn. 2014. Carotenoids, inflammation, and oxidative stress—implications of cellular signaling pathways and relation to chronic disease prevention. Nutrition Research 34 (11): 907–929.
Reuter, S., S.C. Gupta, M.M. Chaturvedi, and B.B. Aggarwal. 2010. Oxidative stress, inflammation, and cancer: how are they linked? Free Radical Biology & Medicine 49 (11): 1603–1616.
Thompson, L.P., and Y. Al-Hasan. 2012. Impact of oxidative stress in fetal programming. Journal of Pregnancy 2012: 1–8.
Zhang, B., Z.-Y. Liu, Y.-Y. Li, Y. Luo, M.-L. Liu, H.-Y. Dong, Y.X. Wang, Y. Liu, P.T. Zhao, F.G. Jin, and Z.C. Li. 2011. Antiinflammatory effects of matrine in LPS-induced acute lung injury in mice. European Journal of Pharmaceutical Sciences 44 (5): 573–579.
Pryor, W.A., and K. Stone. 1993. Oxidants in cigarette smoke radicals, hydrogen peroxide, peroxynitrate, and peroxynitrite. Annals of the New York Academy of Sciences 686 (1): 12–27.
Khodir, A., H. Ghoneim, M. Rahim, and G. Suddek. 2016. Montelukast attenuates lipopolysaccharide-induced cardiac injury in rats. Human & Experimental Toxicology 35 (4): 388–397.
Juranek, I., and S. Bezek. 2005. Controversy of free radical hypothesis: reactive oxygen species-cause or consequence of tissue injury? General Physiology and Biophysics 24 (3): 263–278.
Ben-Shaul, V., L. Lomnitskil, A. Nyska, M. Carbonatto, S. Peano, Y. Zurovskyl, M. Bergman, S.R. Eldridge, and S. Grossman. 2000. Effect of natural antioxidants and apocynin on LPS-induced endotoxemia in rabbit. Human & Experimental Toxicology 19 (11): 604–614.
Chuquimia, O.D., D.H. Petursdottir, M.J. Rahman, K. Hartl, M. Singh, and C. Fernández. 2012. The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems. PLoS One 7 (2): e32125.
Matthay, M.A., and R.L. Zemans. 2011. The acute respiratory distress syndrome: pathogenesis and treatment. Annu Rev Pathol-Mech. 6: 147–163.
Kaspar, J.W., S.K. Niture, and A.K. Jaiswal. 2009. Nrf2: INrf2 (Keap1) signaling in oxidative stress. Free Radical Biology & Medicine 47 (9): 1304–1309.
Kensler, T.W., N. Wakabayashi, and S. Biswal. 2007. Cell survival responses to environmental stresses via the Keap1-Nrf2-ARE pathway. Annual Review of Pharmacology and Toxicology 47: 89–116.
Kim, S.H., J.M. Lee, S.C. Kim, C.B. Park, and P.C. Lee. 2014. Proposed cytotoxic mechanisms of the saffron carotenoids crocin and crocetin on cancer cell lines. Biochemistry and Cell Biology 92 (2): 105–111.
Acknowledgments
The source of data used in this paper was from Ph.D thesis of Mrs. Maryam Kheiry, a student of Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Funding
The authors gratefully acknowledge the help and financial support of the Persian Gulf Physiology Research Center of Ahvaz Jundishapur University of Medical Sciences (No. APRC-9606).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The experiments were carried out in accordance with the ethical guidelines, and the protocol was approved by the Ethics Committee for Animals at Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran (No: IR.AJUMS.REC.1396.275).
Conflict of Interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Kheiry, M., Dianat, M., Badavi, M. et al. p-Coumaric Acid Attenuates Lipopolysaccharide-Induced Lung Inflammation in Rats by Scavenging ROS Production: an In Vivo and In Vitro Study. Inflammation 42, 1939–1950 (2019). https://doi.org/10.1007/s10753-019-01054-6
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10753-019-01054-6