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2,8-Decadiene-1,10-Diol Inhibits Lipopolysaccharide-Induced Inflammatory Responses Through Inactivation of Mitogen-Activated Protein Kinase and Nuclear Factor-κB Signaling Pathway

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Abstract

Amomum tsao-ko (A. tsao-ko) has been used as a traditional medicine for the treatment of infectious and digestive disorders. In the present study, we report the anti-inflammatory activity and molecular mechanism of 2,8-decadiene-1,10-diol (DDO) isolated from the extract of A. tsao-ko in lipopolysaccharide-stimulated RAW 264.7 cells. DDO treatment inhibited the production of nitric oxide and prostaglandin E2 by downregulating inducible nitric oxide synthase and cyclooxygenase-2 expression, respectively. Moreover, DDO suppressed the production of pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor-α. These inhibitory effects of DDO on the expression of inflammatory proteins were found to be mediated through the inactivation of mitogen-activated protein kinases (MAPKs) such as extracellular signal-regulated kinase, c-Jun-N-terminal kinase and p38MAPK, and inhibition of nuclear factor-κB (NF-κB) pathways including degradation of inhibitor of κB-α and nuclear localization of NF-κB. Taken together, these findings demonstrate the pharmacological roles and molecular mechanisms of DDO in regulating inflammatory responses, and suggest further evaluation and development of DDO as a potent therapeutic agent for the treatment of inflammatory disorders.

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Acknowledgments

This study was supported by the research fund of Dankook University in 2015.

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    Authors and Affiliations

    1. Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, 31116, Republic of Korea

      Mi-Sun Kim & Joa Sub Oh

    2. Bio-center, Gyeonggi Institute of Science & Technology Promotion, Suwon, 16229, Republic of Korea

      Eun-Kyung Ahn, Seong Su Hong & Joa Sub Oh

    Authors
    1. Mi-Sun Kim
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    2. Eun-Kyung Ahn
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    3. Seong Su Hong
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    4. Joa Sub Oh
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    Correspondence to Joa Sub Oh.

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    The authors declare that they have no competing interests.

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    Mi-Sun Kim and Eun-Kyung Ahn contributed equally to this work.

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    Kim, MS., Ahn, EK., Hong, S.S. et al. 2,8-Decadiene-1,10-Diol Inhibits Lipopolysaccharide-Induced Inflammatory Responses Through Inactivation of Mitogen-Activated Protein Kinase and Nuclear Factor-κB Signaling Pathway. Inflammation 39, 583–591 (2016). https://doi.org/10.1007/s10753-015-0283-1

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    • DOI: https://doi.org/10.1007/s10753-015-0283-1

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