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Antiasthmatic Effects of Eugenol in a Mouse Model of Allergic Asthma by Regulation of Vitamin D3 Upregulated Protein 1/NF-κB Pathway

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Abstract

The aim of the study was to investigate the antiasthmatic effects of eugenol (EUG) and the possible mechanisms. Asthma model was established by ovalbumin induction. A total of 50 mice were randomly assigned to five experimental groups: control, OVA, OVA + dexamethasone (2 mg/kg), OVA + EUG (10 mg/kg), and OVA + EUG (20 mg/kg). Airway resistance (Raw) were measured, histological studies were evaluated by the hematoxylin and eosin (HE) staining, interleukin-4 (IL-4) and interleukin-5 (IL-5) were evaluated by enzyme-linked immunosorbent assay (ELISA), Vitamin D3 upregulated protein 1 (VDUP1), IκBα, P-IκBα, NF-κBP65, and p-NF-κBP65 were measured by Western blotting. Our study demonstrated that EUG inhibited OVA-induced increases in Raw and eosinophil count; IL-4 and IL-5 were recovered. Histological studies demonstrated that EUG substantially inhibited OVA-induced eosinophilia in the lung tissue. Western blotting studies demonstrated that EUG substantially inhibited P-IκBα, NF-κBP65, and p-NF-κBP65 protein levels and increased VDUP1 and IκBα protein levels. These findings suggest that EUG may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.

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References

  1. Beasley, R., J. Crane, C.K. Lai, and N. Pearce. 2000. Prevalence and etiology of asthma. J Allergy Clin Immunol 105: 466–472.

    Article  Google Scholar 

  2. Busse, W.W., and R.F. Lemanske Jr. 2001. Asthma. N Engl J Med 344: 350–362.

    Article  CAS  PubMed  Google Scholar 

  3. Brightling, C.E., F.A. Symon, S.S. Birring, et al. 2002. TH2 cytokine expression in bronchoalveolar lavage fluid T lymphocytes and bronchial submucosa is a feature of asthma and eosinophilic bronchitis. J Allergy Clin Immunol 110: 899–905.

    Article  CAS  PubMed  Google Scholar 

  4. Robinson, D.S., Q. Hamid, S. Ying, et al. 1992. Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma. N Engl J Med 326: 298–304.

    Article  CAS  PubMed  Google Scholar 

  5. Hershey, G.K. 2003. IL-13 receptors and signaling pathways: an evolving web. J Allergy Clin Immunol 111: 677–690.

    Article  CAS  PubMed  Google Scholar 

  6. Song, H., D. Cho, J.H. Jeon, S.H. Han, D.Y. Hur, Y.S. Kim, and I. Choi. 2003. Vitamin D(3) up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species. Immunol Lett 86: 235–247.

    Article  CAS  PubMed  Google Scholar 

  7. Junn, E., S.H. Han, J.Y. Im, Y. Yang, E.W. Cho, H.D. Um, D.K. Kim, K.W. Lee, P.L. Han, S.G. Rhee, and I. Choi. 2000. Vitamin D3 up-regulated protein 1 mediates oxidative stress via suppressing the thioredoxin function. J Immunol 164: 6287–6295.

    Article  CAS  PubMed  Google Scholar 

  8. Kwon, H.-J., Y.-S. Won, and H.-W. Suh. 2014. Vitamin D3 upregulated protein 1 suppresses TNF-α-induced NF-kB activation in hepatocarcinogenesis. J Immunol 185: 3980–3989.

    Article  Google Scholar 

  9. Ahmad, I., Z. Mehmood, and F. Mohammad. 1998. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol 62(2): 183–193.

    Article  CAS  PubMed  Google Scholar 

  10. Nagababu, E., and N. Lakshmaiah. 1992. Inhibitory effect of eugenol on non-enzymatic lipid peroxidation in rat liver mitochondria. Biochem Pharmacol 43(11): 2393–2400.

    Article  CAS  PubMed  Google Scholar 

  11. Oh, S.R., M.Y. Lee, K. Ahn, B.Y. Park, O.K. Kwon, H. Joung, J. Lee, D.Y. Kim, S. Lee, J.H. Kim, and H.K. Lee. 2006. Suppressive effect of verproside isolated from Pseudolysimachion longifolium on airway inflammation in a mouse model of allergic asthma. Int Immunopharmacol 6: 978–986.

    Article  CAS  PubMed  Google Scholar 

  12. Djukanović, R., W.R. Roche, J.W. Wilson, C.R. Beasley, O.P. Twentyman, R.H. Howarth, and S.T. Holgate. 1990. Mucosal inflammation in asthma. Am J Respir Cirtical Care Med 142: 434–457.

    Google Scholar 

  13. Duan, W., J.H. Chan, C.H. Wong, B.P. Leung, and W.S. Wong. 2004. Anti inflammatory effects of mitogen-activated protein kinase inhibitor U0126 in an asthma mouse model. J Immunol 172: 7053–7059.

    Article  CAS  PubMed  Google Scholar 

  14. Wenzel, S.E. 2006. Asthma: defining of the persistent adult phenotypes. Lancet 368: 804–813.

    Article  CAS  PubMed  Google Scholar 

  15. Elsner, J., and A. Kapp. 1999. Regulation and modulation of eosinophil effector functions. Allergy 54: 15–22.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pediatrics, Huai’an First People’s Hospital, Nanjing Medical University, 6 Beijing Road West, Huai’an, 223300, Jiangsu, People’s Republic of China

    Chenglin Pan & Zewu Dong

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  1. Chenglin Pan
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  2. Zewu Dong
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Correspondence to Zewu Dong.

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Pan, C., Dong, Z. Antiasthmatic Effects of Eugenol in a Mouse Model of Allergic Asthma by Regulation of Vitamin D3 Upregulated Protein 1/NF-κB Pathway. Inflammation 38, 1385–1393 (2015). https://doi.org/10.1007/s10753-015-0110-8

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