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In Vitro Oxidation of Fibrinogen Promotes Functional Alterations and Formation of Advanced Oxidation Protein Products, an Inflammation Mediator

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Abstract

Fibrinogen (FB) is a soluble blood plasma protein and is a key molecule involved in coagulation. Oxidative modification of proteins, such as the formation of advanced oxidation protein products (AOPP), a heterogeneous family of protein compounds structurally modified and derived from oxidative stress, may be associated with the pathophysiology of a number of chronic inflammatory diseases. Therefore, the aim of this study was to determine whether the formation of this mediator of inflammation occurs from FB and whether its generation is associated with structural changes. Results of the present study suggest that the oxidation of FB may provoke the formation of AOPP, which in turn, may promote functional alterations in FB, thus causing changes in its structural domains and increasing its procoagulant activity.

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Acknowledgments

This study was supported by scholarships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil).

Conflict of Interest

There are no conflicts of interest to declare.

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Authors and Affiliations

  1. Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil

    Vanessa Dorneles Torbitz, Guilherme Vargas Bochi, José Antônio Mainardi de Carvalho & Rafael Noal Moresco

  2. Pharmaceutical Sciences Postgraduate Program, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil

    Vanessa Dorneles Torbitz, José Antônio Mainardi de Carvalho, José Edson Paz da Silva & Rafael Noal Moresco

  3. Pharmacology Postgraduate Program, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil

    Guilherme Vargas Bochi & Rafael Noal Moresco

  4. Laboratory of Microbiology, Center of Health Sciences, Franciscan University Center, Santa Maria, RS, Brazil

    Rodrigo de Almeida Vaucher

Authors
  1. Vanessa Dorneles Torbitz
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  2. Guilherme Vargas Bochi
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  3. José Antônio Mainardi de Carvalho
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  4. Rodrigo de Almeida Vaucher
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  5. José Edson Paz da Silva
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  6. Rafael Noal Moresco
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Correspondence to Rafael Noal Moresco.

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Torbitz, V.D., Bochi, G.V., de Carvalho, J.A.M. et al. In Vitro Oxidation of Fibrinogen Promotes Functional Alterations and Formation of Advanced Oxidation Protein Products, an Inflammation Mediator. Inflammation 38, 1201–1206 (2015). https://doi.org/10.1007/s10753-014-0085-x

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  • DOI: https://doi.org/10.1007/s10753-014-0085-x

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