Skip to main content

Advertisement

SpringerLink
Log in

Inflammatory Cytokines Content in Achilles Tendinopathy after Phonophoresis Treatment Combined with Gold Nanoparticles and Diclophenac Diethylammonium in Rats

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Tendinitis is a painful condition that occurs in tendons in response to repetitive use or direct trauma. The therapeutic approaches commonly employed to modulate inflammation have not achieved complete success in chronic cases of tendinitis. In this scenario, considering the anti-inflammatory properties of pulsed therapeutic ultrasound and gold nanoparticles (GNPs), this study assesses the possible therapeutic effects of phonophoresis in association with diclophenac diethylammonium and GNPs by measuring the inflammatory parameters interleukin 1β and tumor necrosis factor alpha in acute tendinous injury. Wistar rats were randomly divided into three groups and were treated with phonophoresis and diclophenac diethylammonium, GNP gel, and a combination thereof. A significant decrease in interleukin 1β and tumor necrosis factor alpha occurred in tendons treated with phonophoresis + diclophenac + GNPs. The content of both cytokines were similar after combined treatment with phonophoresis + diclophenac + GNPs. Apart from the anti-inflammatory effect, GNPs transported and enhanced drug action when used with phonophoresis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

REFERENCES

  1. Wang, J.H.C. 2006. Mechanobiology of tendon. Journal of biomechanics 39: 1563–1582.

    Article  PubMed  Google Scholar 

  2. Yinghua, X., and A.C.M. George. 2008. The basic science of tendinopathy. Clinical Orthopaedcs and Related Research 466: 1528–1538.

    Article  Google Scholar 

  3. Kraychete, D.C., M.T.A. Calasans, and C.M.L. Valente. 2006. Citocinas pró-inflamatórias e dor. Revista Brasileira de Reumatologia 46: 199–206.

    Article  Google Scholar 

  4. Bratt, M., M.D. Andres, A.C. George, et al. 2008. Treatment of tendinopathy. Clinical Orthopaedics and Related Research 466: 1542–1554.

    Google Scholar 

  5. Kitchen, S.S., and C.J. Patridge. 1990. A review of therapeutic ultrasound Part 1: Background and physiological effects. Physiotherapy 76: 593–599.

    Article  Google Scholar 

  6. Merino, G., Y.N. Kalia, and R.H. Guy. 2003. Ultrasound-enhanced transdermal transport. Journal Pharmaceutical Science 92: 1125–1137.

    Article  CAS  Google Scholar 

  7. Currier, D.P., D. Greathouse, and T. Swift. 1978. Sensory nerve conduction: Effect of ultrasound. Archives of Physical Medicine and Rehabilitation 59: 181–185.

    CAS  PubMed  Google Scholar 

  8. Selvakannan, P.R., M.L. Saikat, P. Sumant, et al. 2003. Capping of gold nanoparticles by the amino acid lysine renders them water-dispersible. Langmuir 19: 3545–3549.

    Article  CAS  Google Scholar 

  9. Bronaugh, R.L., and H.I. Maibach. 1991. In vitro percutaneous absorption: Principles, fundamentals, and applications, 1991. Annual Arbor: CRC Press.

    Google Scholar 

  10. Chien, Y.W. 1987. Advances in transdermal systemic medications. In Transdermal-controlled systemic medications: Volume 31, ed. Y.W. Chien, 1–24. New York: Marcel Dekker Inc.

    Google Scholar 

  11. Chien, Y.W. 1987. Developmental concepts and practice in transdermal therapeutic systems. In Transdermal-controlled systemic medications: Volume 31, ed. Y.W. Chien, 25–82. New York: Marcel Dekker Inc.

    Google Scholar 

  12. Dyson, M., J.B. Pond, J. Josefh, and R. Warwic. 1968. The stimulation of tissue regeneration by means of ultrasound. Clinical Science 35: 273–285.

    CAS  PubMed  Google Scholar 

  13. Cunha, A., N.A. Parizotto, and B.C. Vidal. 2001. The effect of therapeutic ultrasound on repair of the Achilles tendon of the rat. Ultrasound Medicine & Biology 27: 1691–1696.

    Article  Google Scholar 

  14. Koeke, P.U., N.A. Parizotto, P.M. Carrinho, et al. 2005. Comparative study of the efficacy of the topical application of hydrocortisone, therapeutic ultrasound and phonophoresis on the tissue repair process in rat tendons. Ultrasound Medicine & Biology 31: 345–350.

    Article  Google Scholar 

  15. Nishihata, T., K. Kotera, Y. Nakano, et al. 1987. Rat percutaneous transport of diclofenac and influence of hydrogenated soya phospholipids. Chemical & Pharmaceutical Bulletin 35: 3807–3812.

    Article  CAS  Google Scholar 

  16. Daniel, M.C., and D. Astruc. 2004. Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size related properties, and applications toward biology, catalysis, and nanotechnology. Chemical Reviews 104: 293–346.

    Article  CAS  PubMed  Google Scholar 

  17. Ghosh, P., G. Han, M. De, et al. 2008. Gold nanoparticles in delivery applications. Advanced Drug Delivery Reviews 60: 1307–1315.

    Article  CAS  PubMed  Google Scholar 

  18. Turkevich, J., P. Stevenson, and J. Hillier. 1951. A study of the nucleation and growth processes in the synthesis of colloidal gold. Discussion Faraday Society 11: 55–60.

    Article  Google Scholar 

  19. Aromal, S.A., and D. Philip. 2012. Green synthesis of gold nanoparticles using Trigonella Foenumgraecum and its size-dependent catalytic activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 97: 1–5.

    Article  Google Scholar 

  20. Rizzi, C.F., J.L. Mauriz, and D.S.F. Corrêa. 2006. Effects of low-level laser therapy (LLLT) on the nuclear factor (NF)-kB signaling pathway in traumatized muscle. Lasers in Surgery and Medicine 38: 704–713.

    Article  PubMed  Google Scholar 

  21. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochemistry 72: 248–254.

    Article  CAS  Google Scholar 

  22. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.

    Article  CAS  PubMed  Google Scholar 

  23. Ka-yun, N.G., and L. Yang. 2002. Therapeutic ultrasound: Its application in drug delivery. Medicine Research Reviews 22: 204–208.

    Article  Google Scholar 

  24. Tsai, C.Y., A.L. Shiau, S.Y. Chen, et al. 2007. Amelioration of collagen-induced arthritis in rats by nanogold. Arthritis Rheumatic 56: 544–554.

    Article  Google Scholar 

  25. Ter Haar, G. 1999. Therapeutic ultrasound. European Journal Ultrasound 1: 93–99.

    Google Scholar 

  26. Pedersen, M.O., A. Larsen, D.S. Pedersen, et al. 2009. Metallic gold reduces TNFα expression, oxidative DNA damage and pro-apoptotic signals after experimental brain injury. Brain Research 1271: 103–113.

    Article  CAS  PubMed  Google Scholar 

  27. Hsich, Y.L. 2006. Effects of ultrasound and diclophenac phonophoresis on inflammatory pain relief: Suppression of inducible nitric oxide synthase in arthritic rats. Physical Therapy 86: 39–49.

    Google Scholar 

  28. Pedersen, D.B., and S. Duncan. 2005. Substituent effects on the adsorption of dialkyl sulfides on gold nanoparticles. Journal Physical Chemistry Annual 15: 11172–11179.

    Article  Google Scholar 

Download references

ACKNOWLEDGMENTS

This work was supported by grants from Conselho Nacional de Pesquisa e Desenvolvimento (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Universidade do Extremo Sul Catarinense (UNESC). The authors would also like to thank CNPq and UNESC for fellowship support.

Author information

Authors and Affiliations

  1. Laboratório de Síntese de Complexos Multifuncionais, Programa de Pós-Graduação em Ciências e Engenharia de Materiais, Universidade do Extremo Sul Catarinense, Av. Universitária, 1105 Universitário, P.O. Box 3167, 88806-000, Criciúma, Santa Catarina, Brazil

    Marcelo B. Dohnert, Gabriela K. Ferreira, Elton Torres Zanoni, Luciana H. Dohnert & Marcos M. S. Paula

  2. Laboratório de Fisiologia e Bioquímica do Exercício, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, 88806-00, Criciúma, Santa Catarina, Brazil

    Paulo Cesar Lock Silveira & Cláudio Teodoro de Souza

Authors
  1. Marcelo B. Dohnert
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gabriela K. Ferreira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paulo Cesar Lock Silveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Elton Torres Zanoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Luciana H. Dohnert
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cláudio Teodoro de Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Marcos M. S. Paula
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marcos M. S. Paula.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dohnert, M.B., Ferreira, G.K., Silveira, P.C.L. et al. Inflammatory Cytokines Content in Achilles Tendinopathy after Phonophoresis Treatment Combined with Gold Nanoparticles and Diclophenac Diethylammonium in Rats. Inflammation 38, 1044–1049 (2015). https://doi.org/10.1007/s10753-014-0069-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-014-0069-x

KEY WORDS

Search

Navigation