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Nuclear inelastic scattering at the diiron center of ribonucleotide reductase from Escherichia coli

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Abstract

The enzyme ribonucleotide reductase R2 catalyzes an important step in the synthesis of the building blocks of DNA, and harbors a dinuclear iron center required for activity. Not only the iron valence states but also the protonation of the iron ligands govern the enzymatic activity of the enzyme. We have performed Nuclear Inelastic Scattering (NIS) experiments on the 57Fe reconstituted ribonucleotide reductase R2 subunit from Escherichia coli (Ec R2a). Accompanying Mössbauer spectroscopic investigations show that the partial density of vibrational states (pDOS) of the 57Fe reconstituted Ec R2a sample contained contributions from both 57Fe-Ec R2a protein as well as unspecifically bound 57Fe. Subtraction of a featureless pDOS as obtained from protein-coated iron oxide particles allowed modeling of the contribution of non-specifically bound iron and thus the pDOS of 57Fe-Ec R2a could be obtained. Quantum-mechanics/molecular-mechanics (QM/MM) calculations of the whole 57Fe-Ec R2a protein with variations of the cofactor protonation were performed in order to assign characteristic bands to their corresponding molecular vibrational modes.

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Acknowledgements

This work has been supported by the German Federal Ministry of Education and Research and the Swedish Research Council via the German-Swedish Röntgen-Ångström-Cluster.

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Authors and Affiliations

  1. Department of Physics, University of Kaiserslautern, Kaiserslautern, Germany

    J. Marx, I. Faus, H. Auerbach, L. Scherthan, K. Jenni & V. Schünemann

  2. Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden

    V. Srinivas & M. Högbom

  3. Nuclear Resonance Group, European Synchrotron Radiation Facility, Grenoble, France

    A. I. Chumakov & R. Rüffer

  4. Department of Physics, Free University of Berlin, Berlin, Germany

    M. Haumann

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  1. J. Marx
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  2. V. Srinivas
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Correspondence to J. Marx.

Additional information

This article is part of the Topical Collection on Proceedings of the International Conference on the Applications of the Mössbauer Effect (ICAME 2017), Saint-Petersburg, Russia, 3–8 September 2017

Edited by Valentin Semenov

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Marx, J., Srinivas, V., Faus, I. et al. Nuclear inelastic scattering at the diiron center of ribonucleotide reductase from Escherichia coli . Hyperfine Interact 238, 82 (2017). https://doi.org/10.1007/s10751-017-1452-4

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  • DOI: https://doi.org/10.1007/s10751-017-1452-4

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