Abstract
CircRNA circ-PRDM5 (PR/SET domain 5) (circ-PRDM5) is overexpressed in age-related cataracts. Nevertheless, the biological role of circ-PRDM5 in posterior capsule opacities (PCO) (a common complication after cataract surgery) is unclear. Human lens epithelial cells SRA01/04 (LECs) were stimulated with TGF-β2 (transforming growth factor beta-2) to mimic the PCO model in vitro. Cell viability, migration, and invasion were determined by MTT, transwell, or wound-healing assays. Protein levels of EMT (epithelial-to-mesenchymal transition) markers and COL1A2 (collagen type I alpha 2 chain) were analyzed by western blotting (WB). Relative expression of circ-PRDM5, miR-92b-3p, and COL1A2 mRNA was analyzed by qRT-PCR. The targeting relationship was confirmed by dual-luciferase reporter and RIP assays. We observed that circ-PRDM5 and COL1A2 were upregulated in PCO tissues and TGF-β2-treated LECs, while miR-92b-3p was downregulated. Both circ-PRDM5 and COL1A2 knockdown impaired TGF-β2-induced LEC migration, invasion, and EMT. Also, circ-PRDM5 could adsorb miR-92b-3p to regulate COL1A2 expression. Furthermore, miR-92b-3p inhibitor offset circ-PRDM5 knockdown-mediated influence on migration, invasion, and EMT of LECs under TGF-β2 stimulation. Also, COL1A2 overexpression overturned the repressive influence of miR-92b-3p mimic on TGF-β2-induced LEC migration, invasion, and EMT. In summary, TGF-β2-induced circ-PRDM5 facilitated LEC migration, invasion, and EMT by adsorbing miR-92b-3p and increasing COL1A2 expression, offering new insights into the development of PCO.
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10735_2021_10053_MOESM1_ESM.tif
Sup. Fig. 1 Western blotting exhibiting the effects of COL1A2 knockdown on E-cadherin and N-cadherin protein levels in TGF-β2-stimulated LECs. **P <0.01, ***P < 0.001, and ****P < 0.0001 (TIFF 132 kb)
10735_2021_10053_MOESM2_ESM.tif
Sup. Fig. 2 The transfection efficiencies of si-circ-PRDM5, circ-PRDM5, si-circ-COL1A2, and COL1A2. (A) QRT-PCR showing the efficiencies of si-circ-PRDM5 and circ-PRDM5. (B) WB exhibiting the efficiencies of si-circ-COL1A2 and COL1A2. ****P < 0.0001 (TIFF 52 kb)
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Huang, P., Hu, Y. & Duan, Y. TGF-β2-induced circ-PRDM5 regulates migration, invasion, and EMT through the miR-92b-3p/COL1A2 pathway in human lens epithelial cells. J Mol Histol 53, 309–320 (2022). https://doi.org/10.1007/s10735-021-10053-7
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DOI: https://doi.org/10.1007/s10735-021-10053-7