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The intricate role of mast cell proteases and the annexin A1-FPR1 system in abdominal wall endometriosis

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Abstract

Endometriosis is a continuous and progressive disease with a poorly understood aetiology, pathophysiology and natural history. This study evaluated the histological differences between eutopic and ectopic endometria (abdominal wall endometriosis) and the expression of mast cell proteases (tryptase and chymase), annexin A1 (ANXA1) and formyl peptide receptor 1 (FPR1). Ectopic endometrium from 18 women with abdominal wall endometriosis and eutopic endometrium from 10 women without endometriosis were obtained. The endometrial samples were analysed by histopathology, immunohistochemistry and ultrastructural immunogold labeling to determine mast cell heterogeneity (tryptase and chymase positive cells) and the expression levels of ANXA1 and FPR1. Histopathological analysis of the endometriotic lesions showed a glandular pattern of mixed differentiation and an undifferentiated morphology with a significant influx of inflammatory cells and a change in mast cell heterogeneity, as evidenced by a significant increase in the number of chymase-positive cells and endogenous chymase expression. The undifferentiated glandular pattern of endometriotic lesions was positively associated with a marked increase and co-localization of ANXA1 and FPR1 in the epithelial cells. In conclusion, the co-upregulated expression of mast cell chymase and ANXA1–FPR1 system in ectopic endometrium suggests their involvement in the development of endometriotic lesions.

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Acknowledgments

This study was supported by FAPESP (2011/00128-1) and CNPq (302768/2010-6). R Paula Junior was supported by CAPES scholarship. The authors thank the resident students of Department of Gynaecology and Obstetrics, São José do Rio Preto School of Medicine (FAMERP) for their crucial help in eutopic endometrium sample collection.

Conflict of interest

The authors have no competing financial interests to disclose in relation to this manuscript.

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Authors and Affiliations

  1. Laboratório de Imunomorfologia, Departamento de Biologia, Universidade Estadual Paulista (UNESP), São José do Rio Preto, São Paulo, 15054-000, Brazil

    Rubens Paula Jr. & Sonia M. Oliani

  2. Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, 15090-000, Brazil

    Antonio H. Oliani, Denise C. M. Vaz-Oliani & Solange C. G. P. D’Ávila

  3. Laboratório de Histologia, Departamento de Morfologia e Genética, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu 740, Ed. Lemos Torres – 3° andar, São Paulo, São Paulo, 04023-900, Brazil

    Cristiane D. Gil

Authors
  1. Rubens Paula Jr.
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  2. Antonio H. Oliani
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  3. Denise C. M. Vaz-Oliani
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  4. Solange C. G. P. D’Ávila
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  5. Sonia M. Oliani
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  6. Cristiane D. Gil
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Correspondence to Cristiane D. Gil.

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Paula, R., Oliani, A.H., Vaz-Oliani, D.C.M. et al. The intricate role of mast cell proteases and the annexin A1-FPR1 system in abdominal wall endometriosis. J Mol Hist 46, 33–43 (2015). https://doi.org/10.1007/s10735-014-9595-y

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  • DOI: https://doi.org/10.1007/s10735-014-9595-y

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