Abstract
The characterization of PR10 proteins has been conducted in many plant species. However, little is known about the role of PvPR10 in response of common bean (Phaseolus vulgaris L.) to stress. Thus, we aimed in this study to identify the members of P. vulgaris PR10 family that are divided into three major subfamilies according to the phylogenetic relationship analyses. The analysis uncovered that tandem duplication was the major force in the expansion of the genes of P. vulgaris PR10 family. Furthermore, the gene structure and motifs examination revealed that genes of PR10 family were relatively conserved during evolution. Likewise, we recognized in the PvPR10 promoter regions several cis-regulatory elements related not only to abiotic and biotic stresses but also to phytohormones. In P. vulgaris, PvPR10 genes exhibited differential expression profiles in many developmental tissues and organs. In response to many abiotic stresses and phytohormones, several PvPR10 genes were highly expressed in leaves and roots of common bean. Taken together, these data provide a foundation for further functional studies of PvPR10 genes that could be used in stress tolerance improvements.
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Abbreviations
- ABA:
-
Abscisic acid
- CSBP:
-
Cytokinin-specific binding proteins
- ET:
-
Ethylene
- ETP:
-
Ethephon
- JA:
-
Jasmonate
- MLP:
-
Major latex proteins
- NCS:
-
(S)-norcoclaurine synthase
- PBP:
-
Phytohormone binding proteins
- PR10:
-
Pathogenesis-related 10
- qRT-PCR:
-
Quantitative real-time PCR
- SA:
-
Salicylic acid
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We are thankful to the Ministry of Higher Education and Scientific Research (Tunisia) for funding the project ‘19PEJC07-26’ and Program Contract CBBC 2019-2022.
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KF, MM, HM and FB conceived and designed the research. KF, HB and MNS performed the bioinformatic analyses. KF and ST performed the experiments. SJ determined the protein 3D-structures. KF wrote the manuscript. FB, MM and HM reviewed the manuscript. All authors approved the manuscript.
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Feki, K., Tounsi, S., Jemli, S. et al. Genome-wide identification of PR10 family members and expression profile analysis of PvPR10 in common bean (Phaseolus vulgaris L.) in response to hormones and several abiotic stress conditions. Plant Growth Regul 102, 279–295 (2024). https://doi.org/10.1007/s10725-023-00997-z
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DOI: https://doi.org/10.1007/s10725-023-00997-z