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Multivariate analysis of volatile profiles in tea plant infested by tea green leafhopper Empoasca onukii Matsuda

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Abstract

The tea green leafhopper (Empoasca onukii Matsuda) is a severe pest for the tea plant (Camellia sinensis (L.) O. Ktze), which significantly reduces the tea yields and quality. Herbivore feeding events lead to the emission of herbivore-induced plant volatiles (HIPVs) to elicit plant defense. To examine the volatile release profiles of tea shoots infested by leafhoppers, gas chromatography-mass spectrometry (GC–MS) analysis was performed. 23 volatiles were emitted in healthy tea shoots whereas 29 volatiles were identified from infested ones. The relative contents of volatiles were significantly increased during infestation. The volatile profiles from healthy and infested tea shoots could be well clustered into two groups by unsupervised clustering analysis and principal component analysis (PCA). Furthermore, a partial least square discriminant analysis (PLS-DA) model can differentiate volatile profiles between healthy and infested tea shoots with a predictive power of 91.8%. From the variable importance for the projection (VIP), eight critical volatiles were identified. Collectively, we have identified a novel volatile release profile after leafhopper infestation in tea plant and provide clues for defensive strategies against herbivores.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work is supported by National Natural Science Foundation of China (Nos. 31971185, 31800316), Key Projects of Support Program for Outstanding Young Talents in Colleges and Universities of Anhui Province (Nos. gxyqZD2020031, gxyq2018034), Key Project of the Education Department of Anhui Province (No. KJ2017A359).

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Author notes

  1. Dan Mu, Chenyang Pan and Zehua Qi have contributed equally to this work.

Authors and Affiliations

  1. The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, School of Life Sciences, Anqing Normal University, NO.1318, North Jixian Road, Anqing, 246133, Anhui, China

    Dan Mu, Zehua Qi, Huaguang Qin, Qin Li, Kexin Liang & Tingzhe Sun

  2. School of Chemistry and Life Sciences, Zhejiang Normal University, Yingbin Road, No.688, Jinhua, 321004, Zhejiang, China

    Chenyang Pan, Qin Li & Yuchun Rao

Authors
  1. Dan Mu
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  2. Chenyang Pan
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  3. Zehua Qi
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  4. Huaguang Qin
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  5. Qin Li
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  6. Kexin Liang
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  7. Yuchun Rao
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  8. Tingzhe Sun
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Contributions

Conceived and designed the experiments: DM, YCR, TZS. Performed the experiments: DM, CYP, ZHQ, QL, KXL. Analyzed the data: DM, CYP, ZHQ, HGQ, TZS. Contributed reagents/materials/analysis tools: YCR, TZS. Wrote the paper: DM, CYP, YCR, TZS.

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Correspondence to Yuchun Rao or Tingzhe Sun.

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Communicated by Daolong Dou.

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Mu, D., Pan, C., Qi, Z. et al. Multivariate analysis of volatile profiles in tea plant infested by tea green leafhopper Empoasca onukii Matsuda. Plant Growth Regul 95, 111–120 (2021). https://doi.org/10.1007/s10725-021-00729-1

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  • DOI: https://doi.org/10.1007/s10725-021-00729-1

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