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Ectopic overexpression of bol-miR390a from broccoli (B. oleracea L var. italica) increases lateral branches in Arabidopsis

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Abstract

MiR390 is a conserved miRNA family and that has been confirmed to play pleiotropic roles in regulating the growth and development of plant species. Broccoli (B. oleracea L var. italica) is an important varietas of B. oleracea with abundant nutrients and cultivated worldwide. However, the mechanism underlying the miR390-mediated regulation of organ development in broccoli remains unknown. In the present study, bol-miR390a, a member of miR390 family, was identified in broccoli. Bol-miR390a displayed significantly differential expression patterns with the development of various broccoli organs. Ectopic overexpression of bol-miR390a accelerated the lateral organ development of transgenic lines in Arabidopsis. The lateral branches and roots of overexpressed bol-miR390a transgenic Arabidopsis were greater than those of the vector controls; thus, the biomass of 35S::bol-miR390a plants significantly increased relative to those of the vector controls. Moreover, the present results demonstrated that the expression levels of TRANS-ACTING SIRNA3 (TAS3)-derived siRNAs (tasiRNAs) displayed positive correlation with those of the bol-miR390a, whereas TAS3 and several auxin response factors (ARFs) including ARF2, ARF3, and ARF4, which were the potential targets of tasiRNAs, showed decreased expression trends in the overexpressed bol-miR390a transgenic plants compared with those in the vector controls. These results suggested that the bol-miR390a/TAS3/ARFs module might play important roles in the lateral organ development of broccoli. In summary, these findings confirmed that bol-miR390a is a crucial regulator in the lateral development of broccoli and suggested the potential application of bol-miR390a in breeding high-biomass crops and economic plants.

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Acknowledgements

This work was funded by grants from the Natural Science Foundation of China (Grant Nos. No. 31872115 and No. 31470669), the National science and technology major special project of transgenes (Grant No. 2018ZX08020003-001-003), the Science and Technology Foundation of Tianjin, China (Grant No. 18ZXZYNC00160) and the State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, China (Grant No. NFUZD2015).

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Authors and Affiliations

  1. College of Life Sciences, Nankai University, Tianjin, China

    Lixia He, Yu Wang, Yinglan Jia, Xue Han, Jiye Yuan, Lihong Li, Chengbin Chen, Wenqin Song & Chunguo Wang

  2. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin, China

    Yaling Yang & Hui Li

  3. Tianjin Union Medical Center, Tianjin, China

    Peng Li

  4. College of Life Sciences, Shandong Normal University, Jinan, Shandong, China

    Min Liu

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  1. Lixia He
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  2. Yu Wang
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  3. Yinglan Jia
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Contributions

LH identified the function assay of bol-miR390a; YW and PL constructed the expression vectors; YJ and YY conducted the genetic transformation; JY and LL conducted the qRT-PCR assay; XH and CC identified the targets of bol-miR390a; WS and ML conducted the phenotypic assay; LH, HL and CW designed the project and wrote the manuscript.

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Correspondence to Hui Li or Chunguo Wang.

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He, L., Wang, Y., Jia, Y. et al. Ectopic overexpression of bol-miR390a from broccoli (B. oleracea L var. italica) increases lateral branches in Arabidopsis. Plant Growth Regul 92, 547–558 (2020). https://doi.org/10.1007/s10725-020-00657-6

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