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Mycorrhizal inoculations and silicon fortifications improve rhizobial symbiosis, antioxidant defense, trehalose turnover in pigeon pea genotypes under cadmium and zinc stress

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Abstract

Cadmium (Cd) is a non-essential and highly toxic element for plant growth while zinc (Zn) becomes toxic at elevated levels. Presence of these heavy metals (HMs) in soils has negative impact on rhizobial symbiosis in legumes leading to reduced agricultural productivity. Role of silicon (Si) amendment and Rhizophagus irregularis in mitigating HM stress has gained importance in recent years. Present study evaluated the individual and cumulative effects of Si and/or AM on Cd (25, 50 mg/kg) or Zn (600, 1000 mg/kg) induced responses in terms of nitrogen fixing efficiency, trehalose biosynthesis, antioxidant defense and phytochelatin (PC) synthesis in pigeon pea genotypes (Tolerant-Pusa 2002, Sensitive-Pusa 991). Results indicated that although mycorrhizal colonization (MC) declined with increase in metal concentration in both genotypes, Pusa 2002 was able to form significant colonization even under stress. Cadmium and zinc stress negatively affected plant biomass and rhizobial symbiosis, with Cd more toxic than Zn. The decline in nodulation potential under both HMs was much more significant in Pusa 991 than Pusa 2002 which could be correlated with proportionately reduced MC, nutrient uptake and ultimate N accumulation. Individual application of AM was much more effective in improving nitrogen fixing efficiency by increasing trehalose biosynthesis, PC production and strengthening antioxidant defense than Si. Restoration of rhizobial symbiosis under combined applications of Si and AM could be correlated with enhanced Si uptake through mycorrhization. Thus, study suggested use of AM as a tool in enhancing benefits of Si nutrition in terms of restoration of nodule senescence and N-fixing competence in pigeon pea under HMs stress.

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Acknowledgements

Financial support provided by Department of Biotechnology (DBT), University Grants Commission (UGC, New Delhi), Government of India in undertaking the present research is gratefully acknowledged. We are also grateful to Pulse laboratory, IARI and TERI (The Energy and Resources Institute), New Delhi for providing the research material.

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  1. Department of Botany, Panjab University, Chandigarh, 160014, India

    Neera Garg & Sandeep Singh

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Garg, N., Singh, S. Mycorrhizal inoculations and silicon fortifications improve rhizobial symbiosis, antioxidant defense, trehalose turnover in pigeon pea genotypes under cadmium and zinc stress. Plant Growth Regul 86, 105–119 (2018). https://doi.org/10.1007/s10725-018-0414-4

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