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Quantitative proteomics analysis of proteins involved in leaf senescence of rice (Oryza sativa L.)

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Abstract

Delaying leaf senescence and prolonging the available time for photosynthesis is one of the important approaches to increase grain yield of rice (Oryza sativa L.). Here, iTRAQ-based quantitative proteomics approach was used to comparative analyze the expression profiles of proteins in rice leaves in response to senescence. Totally 5067 proteins were identified. Compared with the proteins in the flag leaves at early stage of grain-filling in rice Liang-You-Pei 9 (LYP9), 240 and 188 proteins were up-regulated and down-regulated in the flag leaves at middle stage of grain-filling, and 387 and 202 proteins were up-regulated and down-regulated in the flag leaves at late stage of grain-filling, respectively. In addition, 39 and 18 identified proteins were constantly up-regulated and down-regulated in the leaves from early to middle and late stages of grain-filling, respectively. Among them, chloroplast chaperonin 10, geranylgeranyl diphosphate reductase, Mg chelatase subunit ChLD, porphobilinogen deaminase, protochlorophyllide reductase B and thioredoxin-like protein CITRX might have involved in the senescence of leaves. This study provided important information for understanding the age-sensitive mechanism of LYP9, and offered a foundation for future studying and improving it.

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Acknowledgements

This work is supported by the National Key Technology R&D Program (Grant No. 2011BAD35B02-03), the State Key Laboratory of Rice Biology (Grant No. 120104) and the Major Program of the Zhejiang Province for Food Crop Breeding (Grant No. 2016C02050-6).

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    Authors and Affiliations

    1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China

      Xiangbin Xu, Kang Guo, Wenwen Liang, Qifeng Chen, Jun Shi & Bo Shen

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    1. Xiangbin Xu
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    2. Kang Guo
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    3. Wenwen Liang
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    4. Qifeng Chen
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    5. Jun Shi
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    6. Bo Shen
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    Correspondence to Bo Shen.

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    Xiangbin Xu and Kang Guo have contributed equally to this work.

    Electronic supplementary material

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    Table S1 Profile of all the identified proteins in leaves of rice (XLS 11172 KB)

    10725_2017_345_MOESM2_ESM.xls

    Table S2 Profile of the up-regulated proteins in leaves at middle stage of grain-filling of LYP9 compared with that in early stage (XLS 205 KB)

    10725_2017_345_MOESM3_ESM.xls

    Table S3 Profile of the down-regulated proteins in leaves at middle stage of grain-filling of LYP9 compared with that in early stage (XLS 160 KB)

    10725_2017_345_MOESM4_ESM.xls

    Table S4 Profile of the up-regulated proteins in leaves at late stage of grain-filling of LYP9 compared with that in middle stage (XLS 274 KB)

    10725_2017_345_MOESM5_ESM.xls

    Table S5 Profile of the down-regulated proteins in leaves at late stage of grain-filling of LYP9 compared with that in middle stage (XLS 109 KB)

    10725_2017_345_MOESM6_ESM.xls

    Table S6 The constantly up-regulated proteins in the leaves at the early, middle and late stage of grain-filling of LYP9 (XLS 21 KB)

    10725_2017_345_MOESM7_ESM.xls

    Table S7 The constantly down-regulated proteins in the leaves at the early, middle and late stage of grain-filling of LYP9 (XLS 17 KB)

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    Xu, X., Guo, K., Liang, W. et al. Quantitative proteomics analysis of proteins involved in leaf senescence of rice (Oryza sativa L.). Plant Growth Regul 84, 341–349 (2018). https://doi.org/10.1007/s10725-017-0345-5

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    • DOI: https://doi.org/10.1007/s10725-017-0345-5

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