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Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana

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Abstract

Although the importance of zinc finger-containing glycine-rich RNA-binding proteins (RZs) during plant growth and stress adaptation has been determined in diverse plant species, the function and importance of RZs in wheat (Triticum aestivum) are largely unknown. Here, functional role of wheat TaRZ1 in the growth and defense response was evaluated using transgenic Arabidopsis thaliana. Ectopic expression of TaRZ1 in Arabidopsis resulted in retarded plant growth and reduced seed yield under normal growth conditions. Moreover, TaRZ1-expressing transgenic plants displayed increased resistance against the pathogenic bacteria Pseudomonas syringae DC3000. Microarray analysis showed that many genes related to plant growth, metabolism, and stress responses are differently up- or down-regulated in Arabidopsis by TaRZ1 expression. Taken together, these results reveal that wheat TaRZ1 has the ability to inhibit the growth of pathogenic bacteria and Arabidopsis, and suggest that TaRZ1 plays diverse roles in the growth and defense of plants.

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Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen21 Program (PJ00820303), Rural Development Administration and by a grant from the Mid-career Researcher Program (2011-0017357) through the National Research Foundation of Korea grant funded by the Ministry of Education, Science and Technology, Republic of Korea.

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Authors and Affiliations

  1. College of Life Science, Jiangsu Normal University, Xuzhou, 221116, Jiangsu Province, People’s Republic of China

    Tao Xu

  2. Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Korea

    Tao Xu, Hwa Jung Lee, Nguyen Dinh Sy & Hunseung Kang

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  1. Tao Xu
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  2. Hwa Jung Lee
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  4. Hunseung Kang
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Correspondence to Hunseung Kang.

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Xu, T., Lee, H.J., Sy, N.D. et al. Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana . Plant Growth Regul 76, 243–250 (2015). https://doi.org/10.1007/s10725-014-9994-9

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