Abstract
Acer griseum (Franch.) Pax bearing male and female (pseudo-hermaphroditic) flowers on the same individual is a well-known ornamental and endangered plant of China. However, because there is little genetic and sequencing data available, the molecular mechanisms underlying flower development and causes of endangerment in A. griseum are still unclear. In this study, we conducted de novo transcriptome assembly and comparative analysis of female and male flowers at the different developmental stages of A. griseum for the first time. The 163,122,870 qualified reads were acquired and assembled into 224,904 unigenes, of which 161,678 (71.89%) unigenes were annotated in the NR database. By pairwise comparison of unigene abundance between the four samples, many DEGs between the male and female flowers have been detected. Among them, 27 MADS-box genes were identified in A. griseum, phylogenetic analysis divided them into type I (Mα, Mβ, Mγ) and type II subfamilies. Quantitative PCR was applied to validate the DEGs identified in this study. These transcriptome data will be beneficial to further the understanding of the molecular mechanisms regulating the flower development in A. griseum and to lay the foundation for its further breeding.
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Data availability
The sequence data reported in this paper have been deposited in the Genome Sequence Archive in National Genomics Data Center, China National Center for Bioinformation (Accession no: CRA010841) that are publicly accessible at https://ngdc.cncb.ac.cn/gsa. All other data were presented in the article.
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Acknowledgements
This research was funded by the National Natural Science Foundation of China, grant number 31870697; and Investigation and control of alien invasive species at Henan entry ports, grant number 222102110410.
Funding
This work was supported by the National Natural Science Foundation of China, grant number 31870697; and Investigation and control of alien invasive species at Henan entry ports, grant number 222102110410.
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Conceptualization, XZ and YF; methodology, YW, XS, and WF; writing, XZ, and YW; visualization, WF, and XS; funding acquisition, XZ and YF. All authors have read and agreed to the published version of the manuscript.
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Zhou, X., Weng, Y., Fan, Y. et al. Transcriptome characteristics and MADS-box family transcription factors analysis of Acer griseum flowers. Genet Resour Crop Evol (2024). https://doi.org/10.1007/s10722-024-01924-5
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DOI: https://doi.org/10.1007/s10722-024-01924-5