Abstract
The wide geographic distribution, ethno-botanical applications, species identification challenges, and morphological similarities among different species underscore the importance of qualitative analysis in safeguarding botanical purity and ensuring quality control. This study presents a comprehensive characterization of the main commercial plant part, namely the stem, of Tinospora cordifolia, Tinospora sinensis, and Tinospora crispa by considering their physico-chemical characteristics, macroscopic observations, and anatomical details. Through our investigations, we have identified crucial diagnostic features that can aid in distinguishing between different Tinospora species and detecting adulteration in dried raw materials. Notably, T. crispa exhibits more prominent warty protuberances on the stem compared to T. cordifolia and T. sinensis. Furthermore, the transverse section of all three species displays a multilayered cork with distinct radial files and layered cork development. While the heart-shaped leaves of T. cordifolia and T. crispa possess a smooth and pubescent texture respectively. The abaxial surface of T. sinensis leaves is densely coated with silky hairs. To assess the presence of specific bioactive compounds, namely tinosporaside and berberine, we employed high-performance thin-layer chromatography. Our findings reveal that tinosporaside exhibits a band at Rf 0.45 in T. sinensis and T. crispa, whereas all three species display a berberine presence spot with varying intensities at Rf 0.23. These biochemical markers serve as reliable identifiers for species differentiation. Thus, our study establishes a cost-effective approach for enhancing quality control and species identification in Tinospora species, ultimately reducing the occurrence of botanical adulteration.
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References
Ahmad SM, Hoot SB, Qazi PH, Verma V (2009) Phylogenetic patterns and genetic diversity of indian Tinospora species based on chloroplast sequence data and cytochrome P450 polymorphisms. Plant Syst Evol 281(1–4):87–96
Ahmad W, Jantan I, Bukhari SN (2016) Tinospora crispa (L.) Hook. f. and Thomson: a review of its ethnobotanical, phytochemical, and pharmacological aspects. Front Pharm 21(7):59
Anonymous (2023) Plant of World Online, Retrieved from https://powo.science.kew.org/results?f=accepted_names&pagesize=120&q=tinospora. Accessed on 4 July 2023
Begum HJ, Ramamurthy V, Kumar SS (2019) Study of synthesis and characterization of silver nanoparticles from Tinospora cordifolia. Pharm Innov J 8(1):612–615
Bonvicini F, Mandrone M, Antognoni F, Poli F, Gentilomi GA (2014) Ethanolic extracts of Tinospora cordifolia and Alstoniascholaris show antimicrobial activity towards clinical isolates of methicillin-resistant and carbapenemase-producing bacteria. Nat Prod Res 28(18):1438–1445
Choudhary N, Siddiqui MB, Khatoon S (2014) Pharmacognostic evaluation of Tinospora Cordifolia (Willd.) Miers and identification of biomarkers. Ind J Trad Knowl 13:543–550
Chowdhury P (2020) Ayurveda botanicals in COVID-19 management:an in silico multi-target approach. J Biomol Struct Dyn 1–18
Dahanayake JM, Perera PK, Galappatty P, Fernando P, Arawwawala LDAM (2020) Tinospora cordifolia (Willd) Hook. f. (Thomas) grown in Sri Lanka: pharmacognostical, physico-chemical and phytochemical analysis of the stem. J Ayur HerbMed 6(4):217–221
Gowrishankar R, Kumar M, Menon V, Divi SM, Saravanan M, Magudapathy P, Panigrahi BK, Nair KG, Venkataramaniah K (2010) Trace element studies on Tinospora cordifolia (Menispermaceae), Ocimum sanctum (Lamiaceae), Moringa oleifera (Moringaceae), and Phyllanthus niruri (Euphorbiaceae) using PIXE. Bio Trace Ele Res 133(3):357–363
Ilaiyaraja N, Khanum F (2011) Antioxidant potential of Tinospora cordifolia extracts and their protective effect on oxidation of biomolecules. Pharmaco J 3(20):56–62
Kalikar MV, Thawani VR, Varadpande UK, Sontakke SD, Singh RP, Khiyani RK (2008) Immunomodulatory effect of Tinospora cordifolia extract in human immuno-deficiency virus positive patients. Ind J Pharmacol 40(3):107–110
Kapur P, Pereira BMJ, Wuttke W, Jarry H (2009) Androgenic action of Tinospora cordifolia ethanolic extract in prostate cancer cell line LNCaP. Phytomedicine 16(6–7):679–682
Khan MI, Harsha PSCS, Giridhar P, Ravishankar GA (2011) Pigment identification, antioxidant activity, and nutrient composition of Tinospora cordifolia (Willd.) Miers ex Hook. F & Thoms fruit. Inter J Food Sci Nutri 62(3):239–249
Khatoon S, Irshad S, Vijayakumar M, Choudhry N, Siddiqui ZA, Kumar N (2018) Pharmacognostic analysis of Tinospora cordifolia (Thunb.) Miers, with respect to Dioecy. Single Cell Biol 7:1–10
Kumar A, Kumar M, Dandapat S, Sinha MP (2013) Antioxidant activity and pharmacological screening of Tinospora cordifolia (Thunb). Bioscan 8(2):689–693
Lital M, Camelo S, Veillet S, Lafont R, Dilda PJ (2021) Developing new drugs that activate the protective arm of the renin–angiotensin system as a potential treatment for respiratory failure in COVID-19 patients. Drug Discov Today 26(5):1311–1318
Nagral A, Adhyaru K, Rudra OS, Gharat A, Bhandare S (2021) Herbal immune booster-induced liver injury in the COVID-19 pandemic-a case series. J Clin Exp Hepatol 11(6):732–738
Parveen A (2019) Comparative assessment of safety and quality of Tinospora species-Tinospora crispa and Tinospora sinensis Dissertation, The University of Mississippi
Parveen A, Adams JS, Raman V, Budel JM, Zhao J, Babu GN, Khan IA (2020) Comparative morpho-anatomical and HPTLC profiling of Tinospora species and dietary supplements. Planta Med 86(07):470–481
Patani A (ed) (2002) Indian Herbal Pharmacopoeia. Revised new ed. Indian Drug Manufacturers Association, Mumbai
Patel U, Girme A, Patel K, Ghule C, Hingorani L, Gandhi T (2021) A validated HPTLC method for quantification of cordifolioside A, 20-β-hydroxyecdysone and columbin with HPTLC–ESI–MS/MS characterization in stems of Tinospora cordifolia. J Planar Chroma Modern TLC 34(3):217–228
Patturaj R, Ravikumar K (2019) Comparative pharmacognostical and histochemical studies on the three different species of Tinospora on stem and leaf. J Pharm Phytochem 8(2):650–655
Payyappallimana U, Ravikumar K, Venkatasubramanian P (2022) Can Guduchi (Tinospora cordifolia), a well-known ayurvedic hepato-protectant cause liver damage. J Ayur Integ Med 14:100658
Pradhan D, Pandey A (2013) Estimation of starch extracted from different diameter thickness stem of Giloe (Tinospora cordifolia Willd Miers). J Pharmacogn Phytochem 2:162–163
Puratchimani V, Jha S (2007) HPTLC standardization of Tinospora cordifolia using tinosporaside. Ind J Pharm Sci 69(4):578
Qin XY, Luo JY, Gao ZG (2002) Yao ethnic medicinals in China. Ethnic Publish House, Beijing, p 57
Sharma R, Amin H, Prajapati PK (2015) Antidiabetic claims of Tinospora cordifolia (Willd.) Miers: critical appraisal and role in therapy. Asian Pac J Trop Biomed 5(1):68–78
Singh BG, Warrier RR (2004) Tinospora cordifolia. Indian Forester 130:1806
Sivarajan VV, Balachandran I (1999) Ayurvedic drugs and their plant sources. CBS Publishers & Distributors Pvt Ltd, New Delhi, pp 527–544
Solereder H (1908) Systematic anatomy of the Dicotyledons, English Edition. Clarendon Press, Oxford
Srinivasan GV, Unnikrishnan KP, Rema Shree AB, Balachandran I (2008) HPLC estimation of berberine in Tinospora cordifolia and Tinospora sinensis. Ind J Pharma Sci 70:96–99
Tiwari M, Dwivedi UN, Kakkar P (2014) Tinospora cordifolia extract modulates COX-2, iNOS, ICAM-1, pro-inflammatory cytokines and redox status in murine model of asthma. J Ethnopharm 153(2):326–337
Wallis TE A text book of pharmacognosy, reprinted, Metcalfe CR. Chalk L (1950) Anatomy of the dicotyledons 1(2);111–117
World Health Organization (2011) Quality control methods for herbal materials. Publication of World Health Organization
Acknowledgements
Authors express deep gratitude to Shree Dhootapapeshwar Limited, Mumbai, for their valuable support in providing the HPTLC analysis facilities.
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The authors express their gratitude to National Medicinal Plants Board, Ministry of Ayush, Government of India funded Regional Cum Facilitation Centre (RCFC)—Western Region established at Department of Botany, S. P. Pune University, Pune, India for the financial support (Grant Ref No: GOI-A-831).
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Mokat, D.N., Yadav, S. & Benke, A.P. Evaluation of Tinospora species: T. cordifolia, T. sinensis and T. crispa with microscopic imaging, physicochemical properties and major compounds profiling for authentic uses. Genet Resour Crop Evol 71, 1961–1971 (2024). https://doi.org/10.1007/s10722-023-01747-w
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DOI: https://doi.org/10.1007/s10722-023-01747-w