Skip to main content
SpringerLink
Log in

Genome-wide identification of thaumatin-like protein family genes in Panax notoginseng and analysis of their responses to Fusarium solani infection

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Panax notoginseng (Burke) F. H. Chen, which is commonly referred as ''Sanqi'', is the most widely used Chinese medicinal herb. Root rot can have deleterious effects on the success of Sanqi cultivation, including reductions in yield and quality. Thaumatin-like proteins (TLPs) are large, complex proteins in the pathogenesis-related family 5 (PR-5) that play key roles in the responses of plants to environmental stress (e.g., pathogen infection) and other physiological processes. The aim of this study was to identify the TLP gene family members in the genome of Sanqi and characterize their responses to Fusarium solani infection. A total of 20 PnTLP genes were identified in the Sanqi genome, which were phylogenetically divided into 10 subfamilies (Group I-X). Subcellular localization analysis revealed that most of the PnTLP proteins were in the extracellular space, while a few located in chloroplasts. MEME Suite software was used to characterize the structure of PnTLP genes and identify the conserved domains of PnTLP proteins. Analysis of gene duplications revealed that segmental duplication has played a key role in driving the expansion of the PnTLP family. Analysis of collinear relationships revealed 36 homologous genes between Sanqi and Arabidopsis and nine homologous genes between Sanqi and rice. Prediction of the cis-acting elements of the promoters of PnTLP genes indicated that PnTLP genes are involved in biotic, abiotic stress, and hormone induction. Analysis of expression profiles revealed that TLP genes are involved in plant development and the response of plants to fungal infection. qRT-PCR analyses indicated that the expression of PnTLP genes was up-regulated or down-regulated in response to F. solani infection. The results of this study provide new insights on PnTLP genes and their potential roles in the responses of plants to pathogenic fungi. Additional research may be necessary to clarify the role of PnTLP genes identified in our study in disease resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Cao J, Lv Y, Hou Z, Li X, Ding L (2015) Expansion and evolution of thaumatin-like protein (TLP) gene family in six plants. Plant Growth Regul 79:299–307

    Article  Google Scholar 

  • Chen X, Mao Y, Chai W, Yan K, Liang Z, Xia P (2022) Genome-wide identification and expression analysis of MYB gene family under nitrogen stress in Panax notoginseng. Protoplasma 260:189–205

    Article  PubMed  Google Scholar 

  • Dai Z, Dong S, Miao H, Liu X, Han J, Li C, Gu X, Zhang S (2022) Genome-wide identification of TIFY genes and their response to various pathogen infections in cucumber (Cucumis sativus L.). Sci Hortic 295:110814

    Article  CAS  Google Scholar 

  • Deng J, Guan R, Liang T, Su L, Ge F, Cui X, Liu D (2022) Dirigent gene family is involved in the molecular interaction between Panax notoginseng and root rot pathogen Fusarium solani. Ind Crops Prod 178:114544

    Article  CAS  Google Scholar 

  • Deng M, Dong Y, Xu S, Huang S, Zhai X, Fan G (2023) Genome-wide identification and expression of the paulownia Fortunei MADS-Box gene family in response to phytoplasma infection. Genes (basel) 14(3):696

    Article  CAS  PubMed  Google Scholar 

  • Du J, Zhang Q, Hou S, Chen J, Meng J, Wang C, Liang D, Wu R, Guo Y (2022) Genome-wide identification and analysis of the R2R3-MYB gene family in Theobroma cacao. Genes (basel) 13(9):1572

    Article  CAS  PubMed  Google Scholar 

  • Du Y, Amin N, Ahmad N, Zhang H, Zhang Y, Song Y, Fan S, Wang P (2023) Identification of the function of the pathogenesis-related protein GmPR1L in the resistance of soybean to Cercospora sojina hara. Genes (basel) 14(4):920

    Article  CAS  PubMed  Google Scholar 

  • Feng X, Abubakar AS, Chen K, Yu C, Zhu A, Chen J, Gao G, Wang X, Mou P, Chen P (2023) Genome-wide analysis of R2R3-MYB transcription factors in Boehmeria nivea (L.) gaudich revealed potential cadmium tolerance and anthocyanin biosynthesis genes. Front Genet 14:1080909

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Iqbal I, Tripathi RK, Wilkins O, Singh J (2020) Thaumatin-like protein (TLP) gene family in barley: genome-wide exploration and expression analysis during germination. Genes (basel) 11(9):11091080

    Article  Google Scholar 

  • Jesus-Pires CD, Ferreira-Neto JRC, Bezerra-Neto JP, Kido EA, Oliveira Silva RLD, Pandolfi V, Wanderley-Nogueira AC, Binneck E, Costa AFD, Pio-Ribeiro G (2020) Plant Thaumatin-like proteins: function, evolution and biotechnological applications. Curr Protein Pept Sci 21:36–51

    Article  PubMed  Google Scholar 

  • Jia J, Ge N, Wang Q, Zhao L, Chen C, Chen J (2023) Genome-wide identification and characterization of members of the LEA gene family in Panax notoginseng and their transcriptional responses to dehydration of recalcitrant seeds. BMC Genom 24:126

    Article  CAS  Google Scholar 

  • Kaur A, Pati PK, Pati AM, Nagpal AK (2017) In-silico analysis of cis-acting regulatory elements of pathogenesis-related proteins of Arabidopsis thaliana and Oryza sativa. PLoS ONE 12:0184523

    Article  Google Scholar 

  • Koiwa H, Sato F, Yamada Y (1994) Characterization of accumulation of tobacco PR-5 proteins by IEF-immunoblot analysis. Plant Cell Physiol 35:821–827

    Article  CAS  PubMed  Google Scholar 

  • Li Z, Wang X, Cui Y, Qiao K, Zhu L, Fan S, Ma Q (2020a) Comprehensive genome-wide analysis of Thaumatin-like gene family in four cotton species and functional identification of GhTLP19 involved in regulating tolerance to Verticillium dahlia and drought. Front Plant Sci 11:575015

    Article  PubMed  PubMed Central  Google Scholar 

  • Li X, Li S, Qiu B, Zhang Y, Cui X, Ge F, Liu D (2020b) Thaumatin-like protein genes of Panax notoginseng confers resistance to Alternaria panax. Physiol Mol Plant Pathol 112:101537

    Article  CAS  Google Scholar 

  • Li P, Xu Y, Wang K, Guo W, Gu Y, Lyu S, Huang J, Lin H, Huang C, Xu Z (2022a) Genome-wide identification of TLP gene family and their roles in Carya cathayensis Sarg in response to Botryosphaeria dothidea. Front Plant Sci 13:849043

    Article  PubMed  PubMed Central  Google Scholar 

  • Li S, Wang G, Chang L, Sun R, Wu R, Zhong C, Gao Y, Zhang H, Wei L, Wei Y (2022b) Genome-wide identification, characterization, and expression analysis of tubby-like protein (TLP) gene family members in woodland strawberry (Fragaria vesca). Int J Mol Sci 23(19):11961

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Linthorst HJM, Loon LC (1991) Pathogenesis-related proteins of plants. Crit Rev Plant Sci 10:123–150

    Article  CAS  Google Scholar 

  • Liu X, Zhao C, Yang L, Zhang Y, Wang Y, Fang Z, Lv H (2020a) Genome-wide identification, expression profile of the TIFY gene family in Brassica oleracea var. capitata, and their divergent response to various pathogen infections and phytohormone treatments. Genes (basel) 11(2):127

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Cui J, Zhou X, Luan Y, Luan F (2020b) Genome-wide identification, characterization and expression analysis of the TLP gene family in melon (Cucumis melo L.). Genomics 112:2499–2509

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Zheng L, Jin L, Liu Y, Kong Y, Wang Y, Yu T, Chen J, Zhou Y, Chen M (2022) Genome-wide analysis of the soybean TIFY family and identification of GmTIFY10e and GmTIFY10g response to salt stress. Front Plant Sci 13:845314

    Article  PubMed  PubMed Central  Google Scholar 

  • Ning K, Li M, Wei G, Zhou Y, Zhang G, Huai H, Wei F, Chen Z, Wang Y, Done L (2021) Genomic and transcriptomic analysis provide insights into root rot resistance in Panax notoginseng. Front Plant Sci 12:775019

    Article  PubMed  PubMed Central  Google Scholar 

  • Ram C, Danish S, Kesawat MS, Panwar BS, Verma M, Arya L, Yadav S, Sharma V (2022) Genome-wide comprehensive characterization and expression analysis of TLP gene family revealed its responses to hormonal and abiotic stresses in watermelon (Citrullus lanatus). Gene 844:146818

    Article  CAS  PubMed  Google Scholar 

  • Sharma A, Sharma H, Rajput R, Pandey A, Upadhyay SK (2021) Molecular characterization revealed the role of thaumatin-like proteins of bread wheat in stress response. Front Plant Sci 12:807448

    Article  PubMed  Google Scholar 

  • Shatters RG Jr, Boykin LM, Lapointe SL, Hunter WB, Weathersbee AA (2006) Phylogenetic and structural relationships of the PR5 gene family reveal an ancient multigene family conserved in plants and select animal taxa. J Mol Evol 63:12–29

    Article  CAS  PubMed  Google Scholar 

  • Su J, Song S, Wang Y, Zeng Y, Dong T, Ge X, Duan H (2023) Genome-wide identification and expression analysis of DREB family genes in cotton. BMC Plant Biol 23:169

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tao J, Jia H, Wu M, Zhong W, Jia D, Wang Z, Huang C (2022) Genome-wide identification and characterization of the TIFY gene family in kiwifruit. BMC Genom 23:179

    Article  CAS  Google Scholar 

  • Vigers AJ, Wiedemann S, Roberts WK, Legrand M, Selitrennikoff CP, Fritig B (1992) Thaumatin-like pathogenesis-related proteins are antifungal. Plant Sci 83:155–161

    Article  CAS  Google Scholar 

  • Wang P, Yang L, Sun J, Yang Y, Qu Y, Wang C, Liu D, Huang L, Cui X, Liu Y (2021a) Structure and function of rhizosphere soil and root endophytic microbial communities associated with root rot of Panax notoginseng. Front Plant Sci 12:752683

    Article  PubMed  Google Scholar 

  • Wang K, Cheng Y, Yi L, He H, Zhan S, Yang P (2021b) Genome-wide identification of the Tubby-Like Protein (TLPs) family in medicinal model plant Salvia miltiorrhiza. PeerJ 9:11403

    Article  Google Scholar 

  • Xue G, Hu L, Zhu L, Chen Y, Qiu C, Fan R, Ma X, Cao Z, Chen J, Shi J (2023) Genome-wide identification and expression analysis of CCO gene family in Liriodendron chinense. Plants 12(10):1975

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yang Z, Liu G, Zhang G, Yan J, Dong Y, Lu Y, Fan W, Hao B, Lin Y, Li Y (2021) The chromosome-scale high-quality genome assembly of Panax notoginseng provides insight into dencichine biosynthesis. Plant Biotechnol J 19:869–871

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang Y, Yan H, Wei X, Zhang J, Wang H, Liu D (2017) Expression analysis and functional characterization of a pathogen-induced thaumatin-like gene in wheat conferring enhanced resistance to Puccinia triticina. J Plant Interact 12:332–339

    Article  CAS  Google Scholar 

  • Zhang Y, Chen W, Sang X, Wang T, Gong H, Zhao Y, Zhao P, Wang H (2021) Genome-wide identification of the thaumatin-like protein family genes in Gossypium barbadense and analysis of their responses to Verticillium dahliae infection. Plants (basel) 10(12):2647

    Article  CAS  PubMed  Google Scholar 

  • Zhang Y, Miao L, Yang X, Jiang G (2022) Genome-wide characterization and expression of the TLP gene family associated with Colletotrichum gloeosporioides inoculation in Fragaria x ananassa. PeerJ 10:12979

    Article  Google Scholar 

  • Zhao J, Su X (2010) Patterns of molecular evolution and predicted function in thaumatin-like proteins of Populus trichocarpa. Planta 232:949–962

    Article  CAS  PubMed  Google Scholar 

  • Zheng L, Wan Q, Wang H, Guo C, Niu X, Zhang X, Zhang R, Chen Y, Luo K (2022) Genome-wide identification and expression of TIFY family in cassava (Manihot esculenta Crantz). Front Plant Sci 13:1017840

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge the department of Wenshan Miaoxiang Notoginseng Technology, Co., Ltd and Kunming Yingwu Agricultural Science and Technology Co., Ltd., for providing facilities to accomplish this work.

Funding

This work was supported by the Yunnan Academician Expert Workstation (Grant Number: 202305AF150027); Major Science and Technology Special Project of Yunnan Province (Grant Number: 202202AE090025); Yunnan (Kunming) Academician Expert Workstation (Grant Number: YSZJGZZ-2021062); Postgraduate research Innovation Fund Project in Yunnan Normal University (Grant Number: YJSJJ22-A15).

Author information

Authors and Affiliations

  1. Yunnan Provincial Observation and Research Station of Soil Degradation and Restoration for Cultivating Plateau Traditional Chinese Medicinal Plants, Yunnan Normal University, Kunming, 650500, Yunnan, China

    Yun Yang, Huilin Guan & Jingxin Huang

  2. Yunnan Provincial Renewable Energy Engineering Key Laboratory, Yunnan Normal University, Kunming, 650500, Yunnan, China

    Huilin Guan & Jingxin Huang

  3. Wenshan Academy of Agricultural Sciences, Wenshan, 663000, Yunnan, China

    Yun Yang

  4. Kunming Yingwu Agricultural Science and Technology Co., Ltd, KunmingYunnan, 650500, China

    Zecheng Li

  5. Wenshan Miaoxiang Notoginseng Technology Co., Ltd, Wenshan, 663000, Yunnan, China

    Fugang Wei & Shaozhou Yang

  6. School of Energy and Environment Science, Yunnan Normal University, No. 768 Juxian Street, Kunming, 650500, Yunnan, China

    Huilin Guan

Authors
  1. Yun Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Huilin Guan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fugang Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zecheng Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shaozhou Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jingxin Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

YY, HG, and JH conceptualized and designed the experiments; YY, and FW performed the experiments and analyzed the data; YY wrote the paper; HG, YY revised the manuscript. ZL, and SY prepared the materials. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Huilin Guan.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, Y., Guan, H., Wei, F. et al. Genome-wide identification of thaumatin-like protein family genes in Panax notoginseng and analysis of their responses to Fusarium solani infection. Genet Resour Crop Evol 71, 2267–2279 (2024). https://doi.org/10.1007/s10722-023-01736-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-023-01736-z

Keywords

Search

Navigation