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Molecular and morphological evidences for the hybrid origin of Kushui Rose

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Abstract

Natural hybridization has played a significant role in the evolution of the genus Rosa. Kushui Rose (R. sertata × R. rugosa) is a natural hybrid of R. sertata and the cultivated rose R. rugosa. We conducted molecular sequence analysis and a morphological traits examination in the related taxa of the genus to test the hybrid origin of Kushui Rose. Although principal component analysis of the morphological characteristics revealed that Kushui Rose was intermediate between R. rugosa and R. willmottiae, and R. sertata, the unambiguous sequence additivities of the nuclear DNAs (ITS and GAPDH), as well as the incongruence between the nuclear and chloroplast gene trees, revealed that the hybrid origin of Kushui Rose was R. rugosa and R. willmottiae rather than R. sertata. Furthermore, the cluster relationships in the concatenated chloroplast (cpDNA) trees (psbA-trnH, trnL-F, and trnS-G) implied that R. willmottiae and R. rugosa acted as the maternal parent and the paternal parent, respectively, during homoploidization speciation. Here, our research provides a more reasonable orientation for further tracing the origin of this hybrid species and research on its horticultural traits with heterosis.

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Data availability

The datasets generated during the current study are available in the GenBank repository.

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Funding

This work was supported by National Natural Science Foundation of China (31060033). Industry Support Plan Project of Gansu Province Higher Education (2022CYZC-14). Open Fund Project of the State Key Laboratory of Chromosome Engineering, Institute of Genetic Development, Chinese Academy of Sciences (PCCE-KF-2019-06). Science and Technology Program of Gansu Province (22JR5RA169).

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Author notes

  1. Fan Zhao and Ying Wang have contributed to the work equally and should be regarded as co-first authors.

Authors and Affiliations

  1. College of Life Sciences, Northwest Normal University, 967 Anning East Road, Lanzhou, Gansu, China

    Fan Zhao, Ying Wang, Yu-jie Zhang, Kun Sun, Ling-ling Da & Hui Zhang

Authors
  1. Fan Zhao
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  2. Ying Wang
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  3. Yu-jie Zhang
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  4. Kun Sun
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  5. Ling-ling Da
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  6. Hui Zhang
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by YW, FZ, LLD and HZ. The first draft of the manuscript was written by FZ and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ling-ling Da or Hui Zhang.

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Zhao, F., Wang, Y., Zhang, Yj. et al. Molecular and morphological evidences for the hybrid origin of Kushui Rose. Genet Resour Crop Evol 71, 1257–1269 (2024). https://doi.org/10.1007/s10722-023-01688-4

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