Abstract
Flax (Linum usitatissimum L.) is one of the most significant industrial crops due to its oil and fiber, which have a plethora of uses. Because of this, the characterization and introduction/adaptation of flax crops are of great interest. For this reason, we aimed to investigate the genetic diversity and patterns of flax cultivars/genotypes (a 63-cultivar/genotype was used) employing single sequence repeat microsatellite markers via 16 strong amplicon loci, and by estimating their morphological attributes. We determined the quality of the flax, in terms of oil content and composition, and its fiber quality using scanning electron microscopy and strength-linked attributes. For assessment, a series of statistical analyses were carried out in order to cluster and scatter the relevant parameters corresponding to the cultivars/genotypes. Of the assayed flax cultivar/genotypes, Eckendorfi was found to have promising agromorphological values. The average polymorphic information content values were recorded (= 0.689), and the smallest and largest loci with allele dimensions were Lu9(2) and Lu19-Lua613(6) respectively. Overall, the agromorphological attributes were consistent with the microsatellite-based markers in decoding the genetic diversity and population structure of the flax germplasm.
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Abbreviations
- SSR:
-
Simple sequence repeat
- RAPD:
-
Random amplified polymorphic DNA
- ISSR:
-
Inter simple sequence repeat
- AFLP:
-
Amplified fragment length polymorphism
- PUFA:
-
Polyunsaturated fatty acids
- SDG:
-
Secoisolariciresinol diglucoside
- SEM:
-
Scanning electron microscopy
- PCoA:
-
Principal coefficient analysis
- PCA:
-
Principal component analysis
- UPGMA:
-
Arithmetic mean unweighted double group method
- PIC:
-
Polymorphic information content
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Acknowledgements
The present study was derived from the doctoral thesis of Mehmet Zeki Koçak. Co-author of this study, Assoc. Dr. Ahmet Metin Kumlay passed away on 25 Nov. 2021 after a long struggle with cancer. Thank him very much for his contribution to me lives, he was a valuable mentor and friend, and he will be greatly missed. We extend our special thanks to Gholamreza Gohari and Muhittin Kulak for their critiques in improving the paper, to Adnan Aydin for his great support in molecular analysis and to Mustafa Guven Kaysim for statistical analysis.
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The current doctoral thesis study was financially supported by Project Coordination of Igdir University (Turkey) with project number: ZİF1120D02. In this regard, we would like to send our deep thanks to Igdir University (Turkey).
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All authors have significantly contributed in finalising the research. M.Z.K., A.M.K. and M.H.A. designed the experimental set-up. M.Z.K. performed the greenhouse experiments and relevant biochemical analyses. M.Z.K. and A.M.K. performed the molecular analyses and performed the statistical analysis. M.Z.K. analysed the data and wrote the first draft, while the final draft was read and approved by all authors. M.Z.K., A.M.K. and M.H.A. Methodology, Validation, Conceptualization, Supervision, Writing—original draft.
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Koçak, M.Z., Kumlay, A.M. & Alma, M.H. Morphological and molecular characterization of flax (Linum usitatissimum L.) accessions obtained from different locations in Turkey. Genet Resour Crop Evol 70, 2235–2261 (2023). https://doi.org/10.1007/s10722-023-01589-6
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DOI: https://doi.org/10.1007/s10722-023-01589-6