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Population genetic structure of a thousand rubber tree accessions from wild Rondônia populations conserved in Vietnam

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Abstract

Natural rubber is a strategic raw material in many sectors of modern industrial societies and is almost exclusively produced by cultivating rubber trees [Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg.)]. Breeding of this species focuses mainly on advanced improved varieties developed over a century in Southeast Asia and Africa, but it also relies on genetic diversity available in wild germplasm. In this study, we describe the diversity observed within 1060 accessions originating from the state of Rondônia (Brazil) and conserved ex situ in Vietnam. Available passport data for these accessions indicate their putative distribution throughout seven districts in Rondônia. Genotyping data obtained on these accessions with 15 SSR markers generated 458 different alleles. A principal coordinate analysis enabled a clear separation of accessions from Calama district on the first axis, as well as accessions from Ariquemes and Ji-Parana districts on opposite sides of the second axis. A three-step Bayesian analysis of population structure gave a clearer representation, involving three levels of differentiation. At the first differentiation level, the accessions were divided into two clusters, k1 comprising 42% of the accessions, k2 with 25%, and the remaining 33% being admixed accessions. Further levels of Bayesian analysis enabled to distinguish between accessions from different farms in a same district. A better knowledge of the genetic structure at the infra-Rondônia state level, will help in using this available genetic diversity in future breeding programs.

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Data availability

The main dataset analyzed in the present study appears as supplementary material (Matrix_951i_RO_only.xlsx). Other datasets are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to thank the CIRAD and RRIV managements for their facilitating and provision of financial support for this study to be undertaken. Grateful acknowledgements are due to colleagues from CIRAD and from the RRIV Breeding Division for collecting and analyzing the samples. Acknowledgements are also due to Mr. Peter Biggins for correcting the manuscript.

The base map of Rondônia state was kindly provided by Instituto Brasileiro de Geografia e Estatística (https://www.ibge.gov.br).

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Author notes

  1. Lai Van Lam: Deceased.

Authors and Affiliations

  1. Rubber Research Institute of Vietnam (RRIV), Ho Chi Minh City, Vietnam

    Vu Van Truong, Le Mau Thuy, Huynh Duc Dinh & Nguyen Thi Thao

  2. Vietnam Rubber Group (VRG), Ho Chi Minh City, Vietnam

    Lai Van Lam

  3. CIRAD, UMR AGAP, 34398, Montpellier, France

    Ronan Rivallan & Vincent Le Guen

  4. AGAP, Univ Montpellier, CIRAD, INRA, INRIA, Montpellier SupAgro, Montpellier, France

    Ronan Rivallan & Vincent Le Guen

  5. Nong Lam University, Ho Chi Minh City, Vietnam

    Huynh Van Biet

Authors
  1. Vu Van Truong
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  2. Lai Van Lam
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  3. Le Mau Thuy
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  4. Huynh Duc Dinh
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  5. Nguyen Thi Thao
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  6. Ronan Rivallan
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  7. Huynh Van Biet
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  8. Vincent Le Guen
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Correspondence to Vincent Le Guen.

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The authors declare that the outcomes described in this article come from their original research work and were entirely produced by themselves, in accordance with moral and ethical standards, without any plagiarism or false data.

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Vu, V.T., Lai, V.L., Le, M.T. et al. Population genetic structure of a thousand rubber tree accessions from wild Rondônia populations conserved in Vietnam. Genet Resour Crop Evol 67, 475–487 (2020). https://doi.org/10.1007/s10722-019-00843-0

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