Abstract
The genetic diversity was studied of 115 Agropyron cristatum accessions from 17 countries. Tetraploids were the most common (74.8%), followed by diploid (16.3%) and hexaploid (6.9%). We observed a relation between geographic distribution and ploidy level. The tetraploids, the most widespread, were found from Europe through Russia to East Asia. The diploids appeared over the same general range, except in Turkey, Iran and Georgia where no diploid accessions were found. Hexaploid accessions mainly came from a region comprising the east of Turkey, the north of Iran and Georgia. A selection of 71 accessions, including all three ploidy levels, were analyzed by capillary electrophoresis using six wheat simple sequence repeat (SSR) markers. All markers presented high levels of polymorphism, generating 166 different alleles ranging in size between 84 and 256 bp. Based on polymorphic information content values obtained (0.579–0.968), all the SSRs were classified as informative markers (values > 0.5). According to the dendrogram generated, all the A. cristatum accessions were distinctly classified. Diploid, tetraploid and hexaploid accessions are not clearly differentiated from each other on the basis of SSR markers. A field experiment was conducted to morphologically characterize 18 accessions including the three ploidy levels. Significant differences were found between the accessions in spike length, spike width and number of spikelets per spike. All the cytological, molecular, and morphological data demonstrate the high genetic diversity present in A. cristatum, making it a valuable resource for future breeding programs.
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References
Asay KH (1992) Breeding potentials in perennial Triticeae grasses. Hereditas 116:167–173
Asay KH, Dewey DR (1979) Bridging ploidy differences in crested wheatgrass with hexaploid x diploid hybrids. Crop Sci 19:519–523
Asay KH, Jensen KB (1996) Wheatgrasses. In: Moser LE, Buxton DR, Casler MD (eds) Cool-season forage grasses. Agronomy Monograph no. 34, Chap. 22. ASA-CSSA-SSSA, Madison, WI, USA, pp 691–724
Asay KH, Jensen KB, Johnson DA, Chatterton NJ, Hansen WT, Horton WH, Young SA (1995) Registration of ‘Douglas’ crested wheatgrass. Crop Sci 35:1510–1511
Asay KH, Chatterton NJ, Jensen KB, Jones TA, Waldron BL, Horton WH (2003) Breeding improved grasses for semiarid rangelands. Arid Land Res Manag 17:469–478
Botstein G, White RL, Skolnick M, Davis RW (1980) Construction of genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331
Che YH, Li HJ, Yang YP, Yang XM, Li XQ, Li LH (2008) On the use of SSR markers for the genetic characterization of the Agropyron cristatum (L.) Gaertn. in northern China. Genet Resour Crop Evol 55:389–396
Che YH, Yang YP, Yang XM, Li XQ, Li LH (2011) Genetic diversity between ex situ and in situ samples of Agropyron cristatum (L.) Gaertn. based on simple sequence repeat molecular markers. Crop Pasture Sci 62:639–644
Chen Q, Jahier J, Cauderon Y (1989) Production and cytogenetic analysis of BC1, BC2, and BC3 progenies of an intergeneric hybrid between Triticum aestivum (L.) Thell. and tetraploid Agropyron cristatum (L.) Gaertn. Theor Appl Genet 84:698–703
Chen SY, Ma X, Zhang XQ, Huang LK, Zhou JN (2013) Genetic diversity and relationships among accessions of five crested wheatgrass species (Poaceae: Agropyron) based on gliadin analysis. Genet Mol Res 12:5704–5713
Copete A, Cabrera A (2017) Chromosomal location of genes for resistance to powdery mildew in Agropyron cristatum and mapping of conserved orthologous set molecular markers. Euphytica 213:189–297
Dewey DR (1969) Hybrids between tetraploid and hexaploid crested wheatgrass. Crop Sci 9:787–791
Dewey DR (1973) Hybrids between diploid and hexaploid crested wheatgrass. Crop Sci 13:474–477
Dewey DR (1984) The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson JP (ed) Gene manipulation in plant improvement, 16th Stadler Genetics Symposium. Plenum Press, New York, pp 209–279
Dewey DR, Asay KH (1982) Cytogenetic and taxonomic relationships among three diploid crested wheatgrasses. Crop Sci 22:645–650
Döležel J, Greilhuber J, Suda J (2007) Estimation of nuclear DNA content in plants using flow cytometry. Nat Protoc 2:2233–2244
Dong Y, Zhou R, Xu S, Li L, Cauderon Y, Wang R (1992) Desirable characteristics in perennial Triticeae collected in China for wheat improvement. Hereditas 116:175–178
García P, Monte JV, Casanova C, Soler C (2002) Genetic similarities among Spanish populations of Agropyron, Elymus and Thinopyrum, using PCR-based markers. Genet Resour Crop Evol 49:103–109
Gul ZD, Yolcu H, Tan M, Serin Y, Gul I (2013) Yield, quality, and other characteristics of selected lines of crested wheatgrass. J Plant Regist 7:373–377
Guo Q, Meng L, Mao P, Tian X (2014) An assessment of Agropyron cristatum tolerance to cadmium contaminated soil. Biol Plant 58:174–178
Han H, Bai L, Su J, Zhang J, Song L, Gao A, Yang X, Li X, Liu W, Li L (2014) Genetic rearrangements of six wheat-Agropyron cristatum 6P addition lines revealed by molecular markers. PLoS ONE 9:e91066
Hanson WD (1959) Minimum family sizes for the planning of genetic experiments. Agron J 51:711–715
Hsiao C, Asay KH, Dewey DR (1989) Cytogenetic analysis of interspecific hybrids and amphiploids between two diploid crested wheatgrasses Agropyron mongolicum and A. cristatum. Genome 32:1079–1084
Jensen KB, Larson SR, Waldron BL, Asay KH (2005) Cytogenetic and molecular characterization of hybrids between 6x, 4x, and 2x ploidy levels in crested wheatgrass. Crop Sci 46:105–112
Knowles RP (1955) A study of variability in crested wheatgrass. Can J Bot 33:534–546
Limin AE, Fowler DB (1990) An interspecific hybrid and amphiploid produced from Triticum aestivum crosses with Agropyron cristatum and Agropyron desertorum. Genome 33:581–584
Martín A, Cabrera A, Esteban E, Hernández P, Ramirez M, Rubiales D (1999) A fertile amphiploid between diploid wheat (Triticum tauschii) and crested wheatgrass (Agropyron cristatum). Genome 42:519–524
Mattera G, Avila MC, Atienza SG, Cabrera A (2015) Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines. Euphytica 203:165–176
Mellish A, Coulman B (2002) Morphological characteristics of crested wheatgrass populations of diverse origin. Can J Plan Sci 82:693–699
Mellish A, Coulman B, Ferdinandez Y (2002) Genetic relationships among selected crested wheatgrass cultivars and species determined on the basis of AFLP markers. Crop Sci 42:1662–1668
Meng L, Guo Q, Mao P, Tian X (2013) Accumulation and tolerance characteristics of zinc in Agropyron cristatum plants exposed to zinc-contaminated soil. Bull Environ Contam Toxicol 91:298–301
Miller EK, Dyer WE (2002) Phytoremediation of pentachlorophenol in the crested wheatgrass (Agropyron cristatum x desertorum) rhizosphere. Int J Phytoremediation 4:223–238
Murray M, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4326
Ochoa V, Madrid E, Said M, Rubiales D, Cabrera A (2015) Molecular and cytogenetic characterization of a common wheat-Agropyron cristatum chromosome translocation conferring resistance to leaf rust. Euphytica 201:89–95
Ray IM, Ab Frank, Berdahl JD (1997) Genetic variances of agronomic and morphological traits of diploid crested wheatgrass. Crop Sci 37:1503–1507
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Sadasivaiah RS, Weijer J (1981) The origin and meiotic behaviour of hexaploid northern wheatgrass (Agropyron dasystachym). Chromosoma 82:121–132
Said M, Cabrera A (2009) A physical map of chromosome 4Hch from Hordeum chilense containing SSR, STS and EST-SSR molecular markers. Euphytica 167:253–259
Said M, Recio R, Cabrera A (2012) Development and characterisation of structural changes in chromosome 3Hch from Hordeum chilense in common wheat and their use in physical mapping. Euphytica 188:429–440
Soliman MH, Rubiales D, Cabrera A (2001) A fertile amphiploid between durum wheat (Triticum turgidum) and the × Agroticum Amphiploid (Agropyron cristatum × T. tauschii). Hereditas 135:183–186
Soliman MH, Cabrera A, Sillero JC, Rubiales D (2007) Genomic constitution and expression of disease resistance in Agropyron cristatum × durum wheat derivatives. Breed Sci 57:17–21
Tai W, Dewey DR (1966) Morphology, cytology and fertility of diploid and colchicine-induced tetraploid crested wheatgrass. Crop Sci 6:223–226
Vogel KP, Arumuganathan K, Jensen KB (1999) Nuclear DNA content of perennial grasses of the Triticeae. Crop Sci 39:661–667
Wang RR (2011) Agropyron and Psathyrostachys. In: Kole Chittaranjan (ed) Wild crop relatives: genomic and breeding resources, cereals, Chapter 2. Springer, Berlin and Heidelberg, pp 77–108
Yang CT, Fan X, Wang XL, Gu MX, Wang Y, Sha LN, Zhang HQ, Kang HY, Xiao X, Zhou YH (2014) Karyotype analysis of Agropyron cristatum (L.) Gaertner. Caryologia 67:234–237
Yousofi M, Aryavand A (2004) Determination of ploidy levels of some populations of Agropyron cristatum (Poaceae) in Iran by flow cytometry. Iran J Sci Technol Trans A Sci 28:137–144
Yousofi M, Esmaeili M, Otroshy M (2013) Genetic variation among natural populations of Agropyron cristatum (Poaceae) based on SDS-PAGE of seed proteins. Iran J Bot 19:186–193
Zhang J, Zhang J, Liu W, Han H, Lu Y, Yang X, Li L (2015) Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length. Theor Appl Genet 128:1827–1837
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This research was funded by Grant AGL2014-52445-R from the Ministerio de Economía y Competitividad, co-financed by the European Regional Development Fund. The authors declare that they have no conflicts of interest.
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Copete, A., Moreno, R. & Cabrera, A. Characterization of a world collection of Agropyron cristatum accessions. Genet Resour Crop Evol 65, 1455–1469 (2018). https://doi.org/10.1007/s10722-018-0630-9
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DOI: https://doi.org/10.1007/s10722-018-0630-9