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ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts’ surfaces are selectively recognized with recombinant banana lectin

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Abstract

The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host’s cells and antibodies. The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms. Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA. The goal was to define the microorganisms’ surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL. Among the tested lectins most selective binding was observed for RCA120 which preferentially bound Lactobacillus casei DG. Recombinant banana lectin showed specific binding to all of the tested fungal species. The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ß-glucan rich surfaces. The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC50 1.81 μg mL−1 and to P. roqueforti with 1.10 μg mL−1. This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ß-glucan in solutions.

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Acknowledgements

This work is supported by Ministry of Education, Science and Technological Development of the Republic of Serbia (Project No. OI 172049 and III 46009). The authors thank Ivan Minic for graphical assistance.

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Authors and Affiliations

  1. Institute of Virology, Vaccines and Sera, Torlak; Vojvode Stepe 458, Belgrade, 11000, Serbia

    Luka Dragacevic, Danijela Kanazir & Rajna Minic

  2. Department of Bromatology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade, 11221, Serbia

    Brizita Djordjevic

  3. Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 16, Belgrade, 11000, Serbia

    Marija Gavrovic-Jankulovic

  4. Institute for Medical Research, University of Belgrade, Dr Subotica 4 (KCS) POB 39, 11129, Belgrade, 102, Serbia

    Vesna Ilic

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  1. Luka Dragacevic
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Contributions

Rajna Minic, Vesna Ilic and Marija Gavrovic-Jankulovic made substantial contributions to conception and design of the study. Material preparation and data collection was done by Luka Dragacevic and Danijela Kanazir. Data analysis and interpretation was done by all authors. The first draft of the manuscript was written by Rajna Minic and Luka Dragacevic, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Rajna Minic.

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Dragacevic, L., Djordjevic, B., Gavrovic-Jankulovic, M. et al. ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts’ surfaces are selectively recognized with recombinant banana lectin. Glycoconj J 37, 95–105 (2020). https://doi.org/10.1007/s10719-019-09898-8

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