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Structure and biological activities of a hexosamine-rich cell wall polysaccharide isolated from the probiotic Lactobacillus farciminis

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Abstract

Lactobacillus farciminis CIP 103136 is a bacterial strain with recognized probiotic properties. However, the mechanisms underlying such properties have only been partially elucidated. In this study, we isolated and purified a cell-wall associated polysaccharide (CWPS), and evaluated its biological role in vitro. The structure of CWPS and responses from stimulation of (i) human macrophage-like THP-1 cells, (ii) human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR2 or TLR4) and (iii) human colonocyte-like T84 intestinal epithelial cells, upon exposure to CWPS were studied. The structure of the purified CWPS from L. farciminis CIP 103136 was analyzed by nuclear magnetic resonance (NMR), MALDI-TOF-TOF MS, and methylation analyses in its native form and following Smith degradation. It was shown to be a novel branched polysaccharide, composed of linear backbone of trisaccharide repeating units of: [→6αGlcpNAc1 → 4βManpNAc1 → 4βGlcpNAc1→] highly substituted with single residues of αGlcp, αGalp and αGlcpNAc. Subsequently, the lack of pro- or anti-inflammatory properties of CWPS was established on macrophage-like THP-1 cells. In addition, CWPS failed to modulate cell signaling pathways dependent of TLR2 and TLR4 in transfected HEK-cells. Finally, in T84 cells, CWPS neither influenced intestinal barrier integrity under basal conditions nor prevented TNF-α/IFN-γ cytokine-mediated epithelium impairment.

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Acknowledgements

The authors wish to acknowledge Lallemand SA (France) and Lallemand-Institut Rosell (Canada) for providing the L. farciminis strain.

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Authors and Affiliations

  1. CNRS UMR 8576, UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, Univ Lille, 59 000, Lille, France

    Emmanuel Maes, Elisabeth Elass-Rochard & Yann Guérardel

  2. Equipe Biochimie des Produits Aquatiques BPA, Institut Régional Charles Violette EA 7394, USC Anses-ULCO, Université du Littoral-Côte d’Opale, Bassin Napoléon, 62327, Boulogne-sur-mer cedex, France

    Irina Sadovskaya & Thierry Grard

  3. Toxalim (Research Centre in Food Toxicology), INRA, ENVT, INP-Purpan, UPS, Université de Toulouse, Toulouse, France

    Mathilde Lévêque, Vassilia Théodorou & Muriel Mercier-Bonin

  4. Faculté de Médecine Rangueil, Centre de Microscopie Electronique Appliquée à la Biologie (CMEAB), Toulouse Cedex, France

    Bruno Payré

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  1. Emmanuel Maes
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Maes, E., Sadovskaya, I., Lévêque, M. et al. Structure and biological activities of a hexosamine-rich cell wall polysaccharide isolated from the probiotic Lactobacillus farciminis. Glycoconj J 36, 39–55 (2019). https://doi.org/10.1007/s10719-018-09854-y

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