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Immunological characterization of a rigid α-Tn mimetic on murine iNKT and human NK cells

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Abstract

The ability of a rigid α-Tn mimetic (compound 1) to activate murine invariant natural killer T (iNKT) and human natural killer (NK) cells, two subsets of lymphocytes involved in cancer immunesurveillance, was investigated. For this purpose, the mimetic 1 was properly conjugated to a stearic acid containing glycerol-based phospholipid (compound 5) to be presented, in the context of the conserved non polymorphic major histocompatibility complex class I-like molecules (CD1d), to iNKT cells. On the contrary, the mimetic 1 was conjugated to a multivalent peptide-based scaffold (compound 6) to induce NK cell activation.

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Acknowledgements

This work has been supported by COST Action CM1102, Ente Cassa di Risparmio di Firenze. O.R. acknowledge the CNRS, the Université Grenoble Alpes, the “Communauté d’agglomération Grenoble-Alpes Métropole” (Nanobio program), the french Agence Nationale de la Recherche (ANR-12-JS07-0001-01 “VacSyn”) and Labex ARCANE (ANR-11-LABX-0003-01).

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Piemonte Orientale “Amedeo Avogadro”, Largo Donegani 2, 28100, Novara, Italy

    Silvia Fallarini, Alvaro Brittoli & Grazia Lombardi

  2. Université de Lyon, Claude Bernard Lyon 1, 43 bvd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France

    Michele Fiore

  3. CNRS, DCM, Université Grenoble-Alpes, 38000, Grenoble, France

    Olivier Renaudet

  4. Institut Universitaire de France, 103 Boulevard Saint-Michel, 75005, Paris, France

    Olivier Renaudet

  5. Department of Chemistry “Ugo Schiff”, University of Florence, Via della Lastruccia 13, 50019, Sesto Fiorentino, FI, Italy

    Barbara Richichi & Cristina Nativi

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  1. Silvia Fallarini
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  2. Alvaro Brittoli
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Correspondence to Barbara Richichi.

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Fallarini, S., Brittoli, A., Fiore, M. et al. Immunological characterization of a rigid α-Tn mimetic on murine iNKT and human NK cells. Glycoconj J 34, 553–562 (2017). https://doi.org/10.1007/s10719-017-9775-6

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  • DOI: https://doi.org/10.1007/s10719-017-9775-6

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