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GlycoMob: an ion mobility-mass spectrometry collision cross section database for glycomics

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Abstract

Ion mobility mass spectrometry (IM-MS) is a promising analytical technique for glycomics that separates glycan ions based on their collision cross section (CCS) and provides glycan precursor and fragment masses. It has been shown that isomeric oligosaccharide species can be separated by IM and identified on basis of their CCS and fragmentation. These results indicate that adding CCSs information for glycans and glycan fragments to searchable databases and analysis pipelines will increase identification confidence and accuracy. We have developed a freely accessible database, GlycoMob (http://www.glycomob.org), containing over 900 CCSs values of glycans, oligosaccharide standards and their fragments that will be continually updated. We have measured the absolute CCSs of calibration standards, biologically derived and synthetic N-glycans ionized with various adducts in positive and negative mode or as protonated (positive ion) and deprotonated (negative ion) ions.

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Acknowledgments

This work was supported by the Australian National eResearch Collaboration Tools and Resources project [NeCTAR RT016 to M.P.C] and Biotechnology and Biological Sciences Research Council (BBSRC) [BB/L017733/1 to W.S, D.J.H, J.L.P.B], the Free University Berlin and the Max Planck Society.

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Authors and Affiliations

  1. Department of Chemistry, University of Oxford, Oxford, OX1 3QZ, UK

    Weston B. Struwe & Justin L. P. Benesch

  2. Free University Chemistry, Institute of Chemistry and Biochemistry, Takustrasse 3, 14195, Berlin, Germany

    Kevin Pagel

  3. Department of Molecular Physics, Fritz Haber Institute of the Max Planck Society, 14195, Berlin, Germany

    Kevin Pagel

  4. Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK

    David J. Harvey

  5. Biomolecular Frontiers Research Centre, Macquarie University, Sydney, NSW 2109, Australia

    Matthew P. Campbell

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  1. Weston B. Struwe
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  2. Kevin Pagel
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  3. Justin L. P. Benesch
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  4. David J. Harvey
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Correspondence to Weston B. Struwe or Matthew P. Campbell.

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Struwe, W.B., Pagel, K., Benesch, J.L.P. et al. GlycoMob: an ion mobility-mass spectrometry collision cross section database for glycomics. Glycoconj J 33, 399–404 (2016). https://doi.org/10.1007/s10719-015-9613-7

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