Abstract
Tunisia is characterized by the presence of specific seed-propagated apricot (Prunus armeniaca L.) material which is found in the oasis agroecosystems. In order to highlight the genetic diversity, population structure, and demographic history of this germplasm, 33 apricot accessions collected from six different oasis regions in southwestern Tunisia were genotyped using 24 microsatellite markers. A total number of 111 alleles was detected with an average of 4.62 alleles per locus. Bayesian model-based clustering analysis indicated four subdivisions within the collection sampled that corresponded mainly to the geographic origin of the material. The analysis of the 33 accessions using chloroplast markers allowed the identification of 32 haplotypes. Overall, the present study highlighted the high Tunisian apricot’s diversity in the traditional oasis agroecosystems with low genetic differentiation. Understanding the structure of seed-propagated apricot collection is crucial for managing collections in regard to adaptive traits for Arid and Saharan climates as well as for identifying interesting genotypes that can be integrated into international coordinated actions of breeding programs.
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Data availability
The datasets used and/or analyzed during the current study are available in the figshare repository, https://doi.org/10.6084/m9.figshare.14724828.
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The research was supported by a bilateral Franco-Tunisian initiative within the framework of the CMCU Project (05 G0904) as well as the Tunisian Ministry of High Education and Scientific Research (Project LR99ES12).
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HB, NTF, JMA and LK conceived the study. LK contributed to the sampling. HB and MAB collected the data. HB, MAB, CN and NZ analyzed the data. HB wrote the manuscript. All authors read and approved the final manuscript.
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Supplementary file5 (PNG 114 KB)
Figure S1. Description of the four steps for the graphical method allowing determination of optimal K according to Evanno’s parameters.
Supplementary file10 (PNG 115 KB)
Figure S2. Mismatch distribution plot for the 33 apricot seed-propagated accessions included in the analysis.
Supplementary file11 (PNG 446 KB)
Figure S3. Median-joining haplotype network elaborated for the 33 apricot accessions studied using chloroplast markers.
Supplementary file12 (PNG 86 KB)
Figure S4. Unrooted Neighbor-Joining phylogenetic tree based on the cpDNA dataset. Numbers above the branches indicate percentage of bootstrap samples supporting them. Accessions in different colors indicate they were obtained from different oasis regions (Gafsa in green, Tozeur in purple, Nefta in blue, Degache in red, Tamaghza in yellow and Midess in black).
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Bourguiba, H., Batnini, MA., Naccache, C. et al. Chloroplastic and nuclear diversity of endemic Prunus armeniaca L. species in the oasis agroecosystems. Genetica 149, 239–251 (2021). https://doi.org/10.1007/s10709-021-00127-5
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DOI: https://doi.org/10.1007/s10709-021-00127-5